Effect of High Hydrostatic Pressure on Aeromonas hydrophila AH 191 Growth in Milk

dc.creatorDuraes-Carvalho, R
dc.creatorSouza, AR
dc.creatorMartins, LM
dc.creatorSprogis, ACS
dc.creatorBispo, JAC
dc.creatorBonafe, CFS
dc.creatorYano, T
dc.date2012
dc.dateAUG
dc.date2014-07-30T17:12:34Z
dc.date2015-11-26T17:44:26Z
dc.date2014-07-30T17:12:34Z
dc.date2015-11-26T17:44:26Z
dc.date.accessioned2018-03-29T00:26:37Z
dc.date.available2018-03-29T00:26:37Z
dc.identifierJournal Of Food Science. Wiley-blackwell, v. 77, n. 8, n. M417, n. M424, 2012.
dc.identifier0022-1147
dc.identifierWOS:000307385800020
dc.identifier10.1111/j.1750-3841.2012.02819.x
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/64403
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/64403
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1287876
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionExposure to high pressure is an efficient method of bacterial inactivation that is particularly important for reducing the microbial load present in foods. In this study, we examined the high pressure inactivation of Aeromonas hydrophila AH 191, a virulent strain that produces aerolysin, a cytotoxic, enterotoxic, and hemolytic toxin. High pressure treatment (250 MPa for 30 min at 25 degrees C in 0.1 M PBS, pH 7.4) of A. hydrophila grown in milk reduced bacterial viability by at least 9 orders of magnitude. Under these conditions, the enterotoxic, hemolytic, and cytotoxic activities of A. hydrophila culture supernatants were unaltered. These results indicate the need for caution in the use of high pressure for food processing since although truly toxigenic bacteria may be inactivated, their toxins may not be, thus posing a risk to human health. At higher pressure (350 MPa) the inactivation of bacteria was much more effective. Scanning electron microscopy showed a significant decrease in the number of bacteria after higher pressurization (350 MPa for 1 h) and transmission electron microscopy showed irregular shaped bacteria, suggestive of important cell wall and membrane damage, and cytoplasm condensation. Practical Application: High pressure inactivates Aeromonas hydrophila efficiently but is enhanced when combined with moderate temperature (40 degrees C). The biological activities of toxins from this bacterium are unaltered under these conditions.
dc.description77
dc.description8
dc.descriptionM417
dc.descriptionM424
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageen
dc.publisherWiley-blackwell
dc.publisherHoboken
dc.publisherEUA
dc.relationJournal Of Food Science
dc.relationJ. Food Sci.
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectAeromonas hydrophila
dc.subjectbacterial inactivation
dc.subjecthigh pressure
dc.subjecttoxins
dc.subjectPolymerase Chain-reaction
dc.subjectTobacco-mosaic-virus
dc.subjectListeria-monocytogenes
dc.subjectEscherichia-coli
dc.subjectMicrobial Inactivation
dc.subjectCytotoxic Enterotoxin
dc.subjectStaphylococcus-aureus
dc.subjectLow-temperature
dc.subjectDrinking-water
dc.subjectAerolysin Gene
dc.titleEffect of High Hydrostatic Pressure on Aeromonas hydrophila AH 191 Growth in Milk
dc.typeArtículos de revistas


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