Dynamics of remyelination in the brain of adult rats after exposure to ethidium bromide

dc.creatorPereira, LAV
dc.creatorDertkigil, MSJ
dc.creatorGraca, DL
dc.creatorCruz-Hofling, MA
dc.date1998
dc.dateJUL
dc.date2014-07-30T17:05:06Z
dc.date2015-11-26T17:42:32Z
dc.date2014-07-30T17:05:06Z
dc.date2015-11-26T17:42:32Z
dc.date.accessioned2018-03-29T00:24:27Z
dc.date.available2018-03-29T00:24:27Z
dc.identifierJournal Of Submicroscopic Cytology And Pathology. Editrice Compositori Bologna, v. 30, n. 3, n. 341, n. 348, 1998.
dc.identifier1122-9497
dc.identifierWOS:000075101100002
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/63621
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/63621
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1287326
dc.descriptionDiseases of the central nervous system with limited prognosis, as multiple sclerosis, have led to the development of experimental models to study the pathophysiology of such diseases. The present investigation deals with the ethidium bromide (EB) model of demyelination with the objective to study the pathogenesis of encephalic demyelinating lesions. A single 10 mu l injection of 0.1% EB in 0.15 M saline was inoculated in the ventral surface of the pens of adult Wistar rats and after times ranging from 24 h to 30 days, the animals were anaesthetized and perfused with Karnovsky fixative for light and electron microscopy studies. From 3 to 7 days after the EB injection the tissue had developed a spongiotic aspect with intra- and extracellular swelling, demyelinating fibers and a number of necrotic glial cells. By 11 days the reactive phase had begun, and a large number of macrophages had migrated to the foci of the lesion, initiating the absorption of the necrotic tissue. In addition, there were oligodendrocyte-remyelinating nerve fibers and astrocytic gliosis. At 15 days Schwann cells-remyelinating fibers were first seen at the periphery of the lesion while the central area acquired a cystic pattern. The results obtained with the EB-demyelinating model in brain of adult rats showed that 1) remyelination by oligodendrocytes surpassed that by Schwann cells and 2) astrocytic processes were present at the areas remyelinated by the former and absent from those remyelinated by the latter.
dc.description30
dc.description3
dc.description341
dc.description348
dc.languageen
dc.publisherEditrice Compositori Bologna
dc.publisherBologna
dc.publisherItália
dc.relationJournal Of Submicroscopic Cytology And Pathology
dc.relationJ. Submicrosc. Cytol. Pathol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectdemyelination
dc.subjectremyelination
dc.subjectethidium bromide
dc.subjectadult rat
dc.subjectCentral-nervous-system
dc.subjectSchwann-cell Remyelination
dc.subjectFibrillary Acidic Protein
dc.subjectSpinal-cord
dc.subjectDemyelination
dc.subjectOligodendrocytes
dc.subjectInjection
dc.subjectAreas
dc.titleDynamics of remyelination in the brain of adult rats after exposure to ethidium bromide
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución