In vitro inhibition of Streptococci binding to enamel acquired pellicle by Plant Lectins

dc.creatorTeixeira, EH
dc.creatorNapimoga, MH
dc.creatorCarneiro, VA
dc.creatorde Oliveira, TM
dc.creatorCunha, RMS
dc.creatorHavt, A
dc.creatorMartins, JL
dc.creatorPinto, VPT
dc.creatorGoncalves, RB
dc.creatorCavada, BS
dc.date2006
dc.dateJUL
dc.date2014-11-17T21:18:03Z
dc.date2015-11-26T17:42:26Z
dc.date2014-11-17T21:18:03Z
dc.date2015-11-26T17:42:26Z
dc.date.accessioned2018-03-29T00:24:20Z
dc.date.available2018-03-29T00:24:20Z
dc.identifierJournal Of Applied Microbiology. Blackwell Publishing, v. 101, n. 1, n. 111, n. 116, 2006.
dc.identifier1364-5072
dc.identifierWOS:000238438400012
dc.identifier10.1111/j.1365-2672.2006.02910.x
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69393
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/69393
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69393
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1287295
dc.descriptionAim: Initial colonization of the tooth surface by streptococci involves the attachment of these bacteria to adsorbed salivary components of the acquired pellicle. In dental biofilm this adhesion may also involve lectin-like components, present on the surface of the organisms, which bind to complementary carbohydrates on the surface of the tooth. Therefore, this work aimed to evaluate the potential of six lectins, extracted from seeds of Leguminosae family members, to inhibit the adherence of five streptococci species to acquired pellicle in vitro. Methods and Results: The lectins used in this work were extracted from Canavalia easiformis, Canavalia brasiliensis, Dioclea violacea, Dioclea grandiflora, Cratylia floribunda and Vatairea macrocarpa. Fluorescence micrography was employed to visualize the ability of FITC-labeled lectins to attach to acquire pellicle. Adherence inhibition was performed on saliva-coated microtiter plates at which lectins solutions were previously incubated followed by incubation with the oral streptococci. Glucose-mannose specific lectins attached to acquired pellicle with high intensity, while galactose specific lectins, from V. macrocarpa, exhibits low intensity attachment. Conclusions: All lectins were able to inhibit the adherence of the microorganisms tested (p < 0.01). Significance and Impact of the Study: Our results suggest that lectins may be useful in anti adhesion therapeutics.
dc.description101
dc.description1
dc.description111
dc.description116
dc.languageen
dc.publisherBlackwell Publishing
dc.publisherOxford
dc.publisherInglaterra
dc.relationJournal Of Applied Microbiology
dc.relationJ. Appl. Microbiol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectacquired pellicle
dc.subjectadherence
dc.subjectbiofilm
dc.subjectlectins
dc.subjectoral streptococci
dc.subjectOral Streptococci
dc.subjectHydroxyapatite
dc.subjectSpecificity
dc.subjectInfection
dc.subjectAdherence
dc.subjectProteins
dc.subjectSobrinus
dc.subjectSurfaces
dc.subjectMutans
dc.subjectSeeds
dc.titleIn vitro inhibition of Streptococci binding to enamel acquired pellicle by Plant Lectins
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución