Pregnancy-induced chromatin remodeling in the breast of postmenopausal women

dc.creatorRusso, J
dc.creatorSantucci-Pereira, J
dc.creatorde Cicco, RL
dc.creatorSheriff, F
dc.creatorRusso, PA
dc.creatorPeri, S
dc.creatorSlifker, M
dc.creatorRoss, E
dc.creatorMello, MLS
dc.creatorVidal, BC
dc.creatorBelitskaya-Levy, I
dc.creatorArslan, A
dc.creatorZeleniuch-Jacquotte, A
dc.creatorBordas, P
dc.creatorLenner, P
dc.creatorAhman, J
dc.creatorAfanasyeva, Y
dc.creatorHallmans, G
dc.creatorToniolo, P
dc.creatorRusso, IH
dc.date2012
dc.dateSEP 1
dc.date2014-11-17T20:14:12Z
dc.date2015-11-26T17:42:00Z
dc.date2014-11-17T20:14:12Z
dc.date2015-11-26T17:42:00Z
dc.date.accessioned2018-03-29T00:23:51Z
dc.date.available2018-03-29T00:23:51Z
dc.identifierInternational Journal Of Cancer. Wiley-blackwell, v. 131, n. 5, n. 1059, n. 1070, 2012.
dc.identifier0020-7136
dc.identifierWOS:000305756900033
dc.identifier10.1002/ijc.27323
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/80951
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/80951
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/80951
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1287172
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionEarly pregnancy and multiparity are known to reduce the risk of women to develop breast cancer at menopause. Based on the knowledge that the differentiation of the breast induced by the hormones of pregnancy plays a major role in this protection, this work was performed with the purpose of identifying what differentiation-associated molecular changes persist in the breast until menopause. Core needle biopsies (CNB) obtained from the breast of 42 nulliparous (NP) and 71 parous (P) postmenopausal women were analyzed in morphology, immunocytochemistry and gene expression. Whereas in the NP breast, nuclei of epithelial cells were large and euchromatic, in the P breast they were small and hyperchromatic, showing strong methylation of histone 3 at lysine 9 and 27. Transcriptomic analysis performed using Affymetrix HG_U133 oligonucleotide arrays revealed that in CNB of the P breast, there were 267 upregulated probesets that comprised genes controlling chromatin organization, transcription regulation, splicing machinery, mRNA processing and noncoding elements including XIST. We concluded that the differentiation process induced by pregnancy is centered in chromatin remodeling and in the mRNA processing reactome, both of which emerge as important regulatory pathways. These are indicative of a safeguard step that maintains the fidelity of the transcription process, becoming the ultimate mechanism mediating the protection of the breast conferred by full-term pregnancy.
dc.description131
dc.description5
dc.description1059
dc.description1070
dc.descriptionAvon Foundation [02-2008-034]
dc.descriptionNational Institutes of Health [CA006927]
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionAvon Foundation [02-2008-034]
dc.descriptionNational Institutes of Health [CA006927]
dc.descriptionFAPESP [06/0066-8]
dc.languageen
dc.publisherWiley-blackwell
dc.publisherHoboken
dc.publisherEUA
dc.relationInternational Journal Of Cancer
dc.relationInt. J. Cancer
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectpregnancy
dc.subjectbreast differentiation
dc.subjectH3 methylation
dc.subjectnoncoding elements
dc.subjecttranscription factors
dc.subjectMammary-gland
dc.subjectCancer Risk
dc.subjectEpithelial-cells
dc.subjectSplicing Factors
dc.subjectImage-analysis
dc.subjectCyclin L1
dc.subjectDifferentiation
dc.subjectCarcinoma
dc.subjectPluripotency
dc.subjectMouse
dc.titlePregnancy-induced chromatin remodeling in the breast of postmenopausal women
dc.typeArtículos de revistas


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