The value of PCR-RFLP molecular markers for the differentiation of immature stages of two necrophagous flies (Diptera : Calliphoridae) of potential forensic importance

dc.creatorThyssen, PJ
dc.creatorLessinger, AC
dc.creatorAzeredo-Espin, AML
dc.creatorLinhares, AX
dc.date2005
dc.dateSEP-OCT
dc.date2014-11-17T20:02:45Z
dc.date2015-11-26T17:41:58Z
dc.date2014-11-17T20:02:45Z
dc.date2015-11-26T17:41:58Z
dc.date.accessioned2018-03-29T00:23:49Z
dc.date.available2018-03-29T00:23:49Z
dc.identifierNeotropical Entomology. Entomological Society Brasil, v. 34, n. 5, n. 777, n. 783, 2005.
dc.identifier1519-566X
dc.identifierWOS:000233257400009
dc.identifier10.1590/S1519-566X2005000500009
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/72635
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/72635
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/72635
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1287162
dc.descriptionThe identification of insect species involved in corpses decomposition is of particular importance in estimating the post-mortem interval (PMI) in forensic science, since the PMI is based on information about the life cycle of necrophagous insects. However, the identification of some insect species, especially in their immature stages, may be complicated by many factors, even for experienced taxonomists. Species of the same genus such as Hemilucilia segmentaria (Fabricius) and H. semidiaphana (Rondani) (Diptera: Calliphoridae) are morphologically and behaviorally very similar, but differ in their growth and maturation rates. These species are abundant in forests, exclusively necrophagous and, therefore, are of potential medicolegal importance for estimating the PMI in criminal events that would take place in those areas. In this study, we assessed the usefulness of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in the differentiation of these two species. Two specific regions of mitochondrial DNA, the Cytochrome oxidase subunit I (COI) and the control region (CR), were amplified by PCR and digested using restriction endonucleases. The cleavage patterns generated by the endonucleases DraI and SspI were suitable for differentiating the two Hemilucilia species. This method can be helpful for the forensic entomologist in estimating the PMI because it provides a fast identification, also making possible the use of the insect at any life stage, including immature specimens, regardless of the conditions of preservation (dead or live specimens).
dc.description34
dc.description5
dc.description777
dc.description783
dc.languageen
dc.publisherEntomological Society Brasil
dc.publisherLondrina,
dc.publisherBrasil
dc.relationNeotropical Entomology
dc.relationNeotrop. Entomol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectforensic entomology
dc.subjectmitochondrial DNA
dc.subjectHemilucilia segmentaria
dc.subjectHemilucilia semidiaphana
dc.subjectOxidase-i Gene
dc.subjectMitochondrial-dna
dc.subjectSpecies Diptera
dc.subjectControl Region
dc.subjectHuman Corpses
dc.subjectIdentification
dc.subjectEntomology
dc.subjectEvolutionary
dc.subjectDistinction
dc.subjectBlowflies
dc.titleThe value of PCR-RFLP molecular markers for the differentiation of immature stages of two necrophagous flies (Diptera : Calliphoridae) of potential forensic importance
dc.typeArtículos de revistas


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