Cloning, Overexpression, Purification and Preliminary Characterization of Human Septin 8

dc.creatorSouza, TACB
dc.creatorBarbosa, JARG
dc.date2010
dc.dateJUL
dc.date2014-07-30T13:59:55Z
dc.date2015-11-26T17:41:56Z
dc.date2014-07-30T13:59:55Z
dc.date2015-11-26T17:41:56Z
dc.date.accessioned2018-03-29T00:23:46Z
dc.date.available2018-03-29T00:23:46Z
dc.identifierProtein Journal. Springer, v. 29, n. 5, n. 328, n. 335, 2010.
dc.identifier1572-3887
dc.identifierWOS:000279317000005
dc.identifier10.1007/s10930-010-9256-2
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/56110
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/56110
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1287154
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionMammalian septins comprise a family of 14 genes that encode GTP-binding proteins involved in important cellular processes such as cytokinesis and exocytosis. Expression of three different constructs encoding human septin 8 were analyzed and the results show that SEPT8GC, a clone expressing the conserved domain plus C-terminal domain of human septin 8 yields the highest amount of recombinant protein. This protein was purified by affinity chromatography followed by a gel filtration chromatography. CD spectrum of SEPT8GC is characteristic of folded proteins and it presents a transition profile with a T (m) of 54 A degrees C. Fluorescence emission spectra, analytic gel filtration and DLS reflect the sample oligomeric heterogeneity with the predominance of dimers in solution. Homology models indicate clearly that the preferred dimer interface is the one comprising the GTP binding site.
dc.description29
dc.description5
dc.description328
dc.description335
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionAssociacao Brasileira de Tecnologia de Luz Sincrotron (ABTLuS)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFAPESP [2005/05149-6, 1998/14138-2]
dc.languageen
dc.publisherSpringer
dc.publisherNew York
dc.publisherEUA
dc.relationProtein Journal
dc.relationProtein J.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectSEPT8
dc.subjectSeptin 8
dc.subjectPurification
dc.subjectExpression
dc.subjectCell cycle
dc.subjectHomo sapiens
dc.subjectMammalian Septin
dc.subjectCircular-dichroism
dc.subjectDown-syndrome
dc.subjectBrain-tumors
dc.subjectExpression
dc.subjectGene
dc.subjectProtein
dc.subjectFilaments
dc.subjectGtpases
dc.subjectBreast
dc.titleCloning, Overexpression, Purification and Preliminary Characterization of Human Septin 8
dc.typeArtículos de revistas


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