In vitro and in vivo documentation of quantum dots labeled Trypanosoma cruzi-Rhodnius prolixus interaction using confocal microscopy

dc.creatorFeder, D
dc.creatorGomes, SAO
dc.creatorde Thomaz, AA
dc.creatorAlmeida, DB
dc.creatorFaustino, WM
dc.creatorFontes, A
dc.creatorStahl, CV
dc.creatorSantos-Mallet, JR
dc.creatorCesar, CL
dc.date2009
dc.dateDEC
dc.date2014-11-17T19:59:02Z
dc.date2015-11-26T17:41:53Z
dc.date2014-11-17T19:59:02Z
dc.date2015-11-26T17:41:53Z
dc.date.accessioned2018-03-29T00:23:42Z
dc.date.available2018-03-29T00:23:42Z
dc.identifierParasitology Research. Springer, v. 106, n. 1, n. 85, n. 93, 2009.
dc.identifier0932-0113
dc.identifierWOS:000272046200012
dc.identifier10.1007/s00436-009-1631-6
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69334
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/69334
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69334
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1287138
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionSemiconductor quantum dots (QDs) are highly fluorescent nanocrystals markers that allow long photobleaching and do not destroy the parasites. In this paper, we used fluorescent core shell quantum dots to perform studies of live parasite-vector interaction processes without any observable effect on the vitality of parasites. These nanocrystals were synthesized in aqueous medium and physiological pH, which is very important for monitoring live cells activities, and conjugated with molecules such as lectins to label specific carbohydrates involved on the parasite-vector interaction. These QDs were successfully used for the study of in vitro and in vivo interaction of Trypanosoma cruzi and the triatomine Rhodnius prolixus. These QDs allowed us to acquire real time confocal images sequences of live T. cruzi-R. prolixus interactions for an extended period, causing no damage to the cells. By zooming to the region of interest, we have been able to acquire confocal images at the three to four frames per second rate. Our results show that QDs are physiological fluorescent markers capable to label living parasites and insect vector cells. QDs can be functionalized with lectins to specifically mark surface carbohydrates on perimicrovillar membrane of R. prolixus to follow, visualize, and understand interaction between vectors and its parasites in real-time.
dc.description106
dc.description1
dc.description85
dc.description93
dc.descriptionFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.descriptionOC-FIOCRUZ
dc.descriptionRio de Janeiro and Centro de Pesquisa em Optica e Fotonica
dc.descriptionCEPOF
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundacao de Ampara Pesquisa do Estado do Rio de Janeiro [FAPERJ E-26/171.111/2005]
dc.languageen
dc.publisherSpringer
dc.publisherNew York
dc.publisherEUA
dc.relationParasitology Research
dc.relationParasitol. Res.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectTriatoma-infestans
dc.subjectLectin
dc.subjectCells
dc.subjectHemolymph
dc.subjectVector
dc.subjectFluoroimmunoassays
dc.subjectErythrocytes
dc.subjectRecognition
dc.subjectMembrane
dc.subjectMultiple
dc.titleIn vitro and in vivo documentation of quantum dots labeled Trypanosoma cruzi-Rhodnius prolixus interaction using confocal microscopy
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución