Comparative studies focusing on transgenic through cp4EPSPS gene and non-transgenic soybean plants: An analysis of protein species and enzymes

dc.creatorArruda, SCC
dc.creatorBarbosa, HS
dc.creatorAzevedo, RA
dc.creatorArruda, MAZ
dc.date2013
dc.date44136
dc.date2014-07-30T14:00:00Z
dc.date2015-11-26T17:41:43Z
dc.date2014-07-30T14:00:00Z
dc.date2015-11-26T17:41:43Z
dc.date.accessioned2018-03-29T00:23:31Z
dc.date.available2018-03-29T00:23:31Z
dc.identifierJournal Of Proteomics. Elsevier Science Bv, v. 93, n. 107, n. 116, 2013.
dc.identifier1874-3919
dc.identifier1876-7737
dc.identifierWOS:000328803300009
dc.identifier10.1016/j.jprot.2013.05.039
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/56142
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/56142
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1287086
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionThis work evaluates the activity of a few key enzymes involved in combating reactive oxygen species (ROS), such as ascorbate peroxidase (EC 1.11.1.11), catalase (EC 1.11.1.6), glutathione reductase (EC 1.6.4.2), and superoxide dismutase (EC 1.15.1.1), as well as the concentration of malondialdehyde and hydrogen peroxide in transgenic and non-transgenic soybean leaves. Additionally, differential protein species from leaves of both genotypes were evaluated by applying a regulation factor of >= 1.8 to further corroborate the hypothesis that genetic modification itself can be a stress factor for these plants. For this task, transgenic soybean plants were obtained from seeds modified with the cp4EPSPS gene. The results revealed higher activities of all evaluated enzymes in transgenic than in non-transgenic soybean leaves (ranging from 13.8 to 70.1%), as well as higher concentrations of malondialdehyde and hydrogen peroxide in transgenic soybean leaves, clearly indicating a condition of oxidative stress established in the transgenic genotype. Additionally, 47 proteins were differentially abundant when comparing the leaves of both plants, with 26 species accurately identified, including the protein involved in the genetic modification (CP4EPSPS). From these results, it is possible to conclude that the plant is searching for a new equilibrium to maintain its metabolism because the stress condition is being maintained within levels that can be tolerated by the plant. Biological significance The present paper is the first one in the literature where are shown translational aspects involving plant stress and the genetic modification for soybean involving the cp4 EPSPS gene. The main biological importance of this work is to make possible the demystification of the genetic modification, allowing answers for some questions that still remain unknown, and enlarge our knowledge about genetically modified organisms. This article is part of a Special Issue entitled: Translational Plant Proteomics. (C) 2013 Elsevier B.V. All rights reserved.
dc.description93
dc.descriptionSI
dc.description107
dc.description116
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFAPESP [09/54676-0, 10/50204-3]
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationJournal Of Proteomics
dc.relationJ. Proteomics
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectSoybean
dc.subjectGenetically modified organisms
dc.subjectcp4EPSPS
dc.subject2-D DIGE
dc.subjectMass spectrometry
dc.subjectOxidative stress
dc.subject2-dimensional Gel-electrophoresis
dc.subjectOxidative Stress
dc.subjectTomato Mutants
dc.subjectSeeds
dc.subjectMetallomics
dc.subjectProteomics
dc.subjectExpression
dc.subjectTolerance
dc.subjectCells
dc.titleComparative studies focusing on transgenic through cp4EPSPS gene and non-transgenic soybean plants: An analysis of protein species and enzymes
dc.typeArtículos de revistas


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