Cytotoxicity of prodigiosin and benznidazole on V79 cells

dc.creatorMelo, PD
dc.creatorDuran, N
dc.creatorHaun, M
dc.date2000
dc.dateAUG 16
dc.date2014-12-02T16:31:06Z
dc.date2015-11-26T17:41:39Z
dc.date2014-12-02T16:31:06Z
dc.date2015-11-26T17:41:39Z
dc.date.accessioned2018-03-29T00:23:27Z
dc.date.available2018-03-29T00:23:27Z
dc.identifierToxicology Letters. Elsevier Ireland Ltd, v. 116, n. 3, n. 237, n. 242, 2000.
dc.identifier0378-4274
dc.identifier1879-3169
dc.identifierWOS:000089397400009
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/57645
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/57645
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/57645
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1287069
dc.descriptionThe cytotoxicity of prodigiosin, an antibiotic and potential trypanocide produced by Serratia marcescens, and Benznidazole, a trypanocidal drug, were assayed on V79 fibroblast cell line. Three independent endpoints for cytotoxicity were evaluated; namely, the nucleic acid content (NAC), MTT reduction and neutral red uptake (NRU). IC50 values of 1-20 mu M were obtained for prodigiosin in the NRU, MTT and NAC tests. Prodigiosin had greater trypanocidal activity (IC50=5 mu M) than Nifurtimox (IC50 = 150 mu M) a known trypanocide drug used in Chagas' disease therapy. Benznidazole was less toxic (IC50 = 2000 mu M) than prodigiosin (IC50 = 1-20 mu M) in V79 cells based on the MTT and NAC assays. Benznidazole stimulated the NRU until 2 mM. Indeed, the cell viability measured with the NRU was higher at all concentrations of benznidazole tested than that measured by MTT reduction and NAC assays. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
dc.description116
dc.description3
dc.description237
dc.description242
dc.languageen
dc.publisherElsevier Ireland Ltd
dc.publisherClare
dc.publisherIrlanda
dc.relationToxicology Letters
dc.relationToxicol. Lett.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectprodigiosin
dc.subjectbenznidazole
dc.subjectChagas' disease
dc.subjectcytotoxicity
dc.subjectV79 fibroblasts MTT
dc.subjectneutral red
dc.subjectRapid Colorimetric Assay
dc.subjectCyto-toxicity Assays
dc.subjectNeutral Red Uptake
dc.subjectTrypanosoma-cruzi
dc.subjectIn-vitro
dc.subjectSerratia-marcescens
dc.subjectCellular Growth
dc.subjectRat Hepatocytes
dc.subjectChagas-disease
dc.subjectChemotherapy
dc.titleCytotoxicity of prodigiosin and benznidazole on V79 cells
dc.typeArtículos de revistas


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