Role of cyclic GMP on inhibition by nitric oxide donors of human eosinophil chemotaxis in vitro

dc.creatorThomazzi, SM
dc.creatorMoreira, J
dc.creatorMarcondes, S
dc.creatorDe Nueci, G
dc.creatorAntunes, E
dc.date2004
dc.dateFEB
dc.date2014-11-17T18:38:57Z
dc.date2015-11-26T17:41:25Z
dc.date2014-11-17T18:38:57Z
dc.date2015-11-26T17:41:25Z
dc.date.accessioned2018-03-29T00:23:12Z
dc.date.available2018-03-29T00:23:12Z
dc.identifierBritish Journal Of Pharmacology. Nature Publishing Group, v. 141, n. 4, n. 653, n. 660, 2004.
dc.identifier0007-1188
dc.identifierWOS:000220379200013
dc.identifier10.1038/sj.bjp.0705661
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/71211
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/71211
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/71211
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1287006
dc.description1 This study was designed to investigate the effects of the nitric oxide (NO) donors sodium nitroprusside (SNP), 3-morpholinosydnonimine (SIN-1) and S-nitroso-N-acetylpenicillamine (SNAP) on N-formyl-L-methionyl-L-leucyl-phenylalanine (fMLP, 1 x 10(-7) M)-induced human eosinophil chemotaxis, cyclic guanosine- 3', 5'-monophosphate (cGMP) levels, protein nitration and cytotoxicity. 2 Human eosinophils were exposed to SNP, SIN-1 and SNAP (0.001-1.0 mM) for either short (10 min) or prolonged (90 min) time periods. Exposition of eosinophils with these NO donors significantly inhibited the eosinophil chemotaxis irrespective of whether cells were exposed to these agents for 10 or 90 min. No marked differences were detected among them regarding the profile of chemotaxis inhibition. 3 Exposition of eosinophils to SNP, SIN-1 and SNAP (0.001-1.0 mm) markedly elevated the cGMP levels above basal levels, but the 90-min exposition resulted in significantly higher levels compared with the 10-min protocols (5.3 +/- 0.6 and 2.6 +/- 0.2 nm 1.5 x 10(6) cells(-1), respectively). The cGMP levels achieved with SNAP were greater than SNP and SIN-1. 4 The NO donors did not induce cell toxicity in any experimental condition used. Additionally, eosinophils exposed to SNP, SIN-1 and SNAP (1.0 mM each) either for 10 or 90 min did not show any tyrosine nitration in conditions where a strong nitration of bovine serum albumin was observed. 5 Our findings show that inhibitory effects of FMLP-induced human eosinophil chemotaxis by NO donors at short or prolonged exposition time were accompanied by significant elevations of cGMP levels. However, additional elevations of cGMP levels do not change the functional profile (chemotaxis inhibition) of stimulated eosinophils.
dc.description141
dc.description4
dc.description653
dc.description660
dc.languageen
dc.publisherNature Publishing Group
dc.publisherLondon
dc.publisherInglaterra
dc.relationBritish Journal Of Pharmacology
dc.relationBr. J. Pharmacol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectcGMP levels
dc.subjectchemotaxis
dc.subjectcytotoxicity
dc.subjecteosinophil
dc.subjectprotein nitration
dc.subjectnitric oxide
dc.subjectSIN-1
dc.subjectSNAP
dc.subjectsodium nitroprusside
dc.subjectPolymorphonuclear Leukocytes
dc.subjectNitrotyrosine Formation
dc.subjectS-nitrosoglutathione
dc.subjectNeutrophil Migration
dc.subjectAirway Inflammation
dc.subjectEnzyme Immunoassays
dc.subjectMonocyte Chemotaxis
dc.subjectProtein Nitration
dc.subjectMice
dc.subjectAdhesion
dc.titleRole of cyclic GMP on inhibition by nitric oxide donors of human eosinophil chemotaxis in vitro
dc.typeArtículos de revistas


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