Vascular dermatan sulfate regulates the antithrombotic activity of heparin cofactor II

dc.creatorHe, L
dc.creatorGiri, TK
dc.creatorVicente, CP
dc.creatorTollefsen, DM
dc.date2008
dc.dateAPR 15
dc.date2014-11-17T18:36:34Z
dc.date2015-11-26T17:41:18Z
dc.date2014-11-17T18:36:34Z
dc.date2015-11-26T17:41:18Z
dc.date.accessioned2018-03-29T00:23:05Z
dc.date.available2018-03-29T00:23:05Z
dc.identifierBlood. Amer Soc Hematology, v. 111, n. 8, n. 4118, n. 4125, 2008.
dc.identifier0006-4971
dc.identifierWOS:000255134700035
dc.identifier10.1182/blood-2007-12-127928
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/72678
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/72678
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/72678
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1286971
dc.descriptionHeparin cofactor II (HCII)-deficient mice form occlusive thrombi more rapidly than do wild-type mice following injury to the carotid arterial endothelium. Dermatan sulfate (IDS) and heparan sulfate (HS) increase the rate of inhibition of thrombin by HCII in vitro, but it is unknown whether vascular glycosaminoglycans play a role in the antithrombotic effect of HCII in vivo. In this study, we found that intravenous injection of either wild-type recombinant HCII or a variant with low affinity for HS (K173H) corrected the abnormally short thrombosis time of HCII-deficient mice, while a variant with low affinity for DS (R189H) had no effect. When HCII was incubated with frozen sections of the mouse carotid artery, it bound specifically to DS in the adventitia. HCII was undetectable in the wall of the uninjured carotid artery, but it became concentrated in the adventitia following endothelial injury. These results support the hypothesis that HCII interacts with DS in the vessel wall after disruption of the endothelium and that this interaction regulates thrombus formation in vivo.
dc.description111
dc.description8
dc.description4118
dc.description4125
dc.languageen
dc.publisherAmer Soc Hematology
dc.publisherWashington
dc.publisherEUA
dc.relationBlood
dc.relationBlood
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectDeficient Mice
dc.subjectEmbryonic Lethality
dc.subjectTissue Factor
dc.subjectThrombosis
dc.subjectBinding
dc.subjectHemostasis
dc.subjectActivation
dc.subjectExpression
dc.subjectAffinity
dc.subjectPlasma
dc.titleVascular dermatan sulfate regulates the antithrombotic activity of heparin cofactor II
dc.typeArtículos de revistas


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