Extraction of phenolic compounds from pitanga (Eugenia uniflora L.) leaves by sequential extraction in fixed bed extractor using supercritical CO2, ethanol and water as solvents

dc.creatorGarmus, TT
dc.creatorPaviani, LC
dc.creatorQueiroga, CL
dc.creatorMagalhaes, PM
dc.creatorCabral, FA
dc.date2014
dc.dateFEB
dc.date2014-07-30T17:34:15Z
dc.date2015-11-26T17:40:56Z
dc.date2014-07-30T17:34:15Z
dc.date2015-11-26T17:40:56Z
dc.date.accessioned2018-03-29T00:22:40Z
dc.date.available2018-03-29T00:22:40Z
dc.identifierJournal Of Supercritical Fluids. Elsevier Science Bv, v. 86, n. 4, n. 14, 2014.
dc.identifier0896-8446
dc.identifier1872-8162
dc.identifierWOS:000333511100002
dc.identifier10.1016/j.supflu.2013.11.014
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/66586
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/66586
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1286864
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionWith the goal of maximizing the extraction yield of phenolic compounds from pitanga leaves (Eugenia uniflora L), a sequential extraction in fixed bed was carried out in three steps at 60 degrees C and 400 bar, using supercritical CO2 (non-polar) as solvent in a first step, followed by ethanol (polarity: 5.2) and water (polarity: 9.0) in a second and third steps, respectively. All extracts were evaluated for global extraction yield, concentration and yield of both polyphenols and total flavonoids and antioxidant activity by DPPH method (in terms of EC50). The nature of the solvent significantly influenced the process, since the extraction yield increased with solvent polarity. The aqueous extracts presented higher global extraction yield (22%), followed by ethanolic (16%) and supercritical extracts (5%). The study pointed out that the sequential extraction process is the most effective in terms of global extraction yield and yield of polyphenols and total flavonoids, because it produced the more concentrated extracts on phenolic compounds, since the supercritical ethanolic extract presented the highest phenolics content (240.5 mg GAE/g extract) and antioxidant capacity (EC50 = 9.15 mu g/mL). The most volatile fraction from the supercritical extract, which is similar to the essential oils obtained by steam distillation or hydrodistillation, presented as major compounds the germacrenos D and B + bicyclogermacrene (40.75%), selina-13,7(11)-trien-8-one + selina-1,3,7(11)-trien-8-one epoxide (27.7%) and trans-caryophyllene (14.18%). (C) 2013 Elsevier B.V. All rights reserved.
dc.description86
dc.description4
dc.description14
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFAPESP [2011/14309-8]
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationJournal Of Supercritical Fluids
dc.relationJ. Supercrit. Fluids
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectEugenia uniflora L.
dc.subjectSupercritical extraction
dc.subjectPhenolic compounds
dc.subjectSequential extraction
dc.subjectFixed bed extractor
dc.subjectAntioxidants
dc.subjectEssential Oil
dc.subjectAntioxidant
dc.subjectPlants
dc.subjectAssays
dc.titleExtraction of phenolic compounds from pitanga (Eugenia uniflora L.) leaves by sequential extraction in fixed bed extractor using supercritical CO2, ethanol and water as solvents
dc.typeArtículos de revistas


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