Molecular evidence that sugarcane yellow leaf virus (ScYLV) is a member of the Luteoviridae family

dc.creatorMaia, IG
dc.creatorGoncalves, MC
dc.creatorArruda, P
dc.creatorVega, J
dc.date2000
dc.date2014-07-30T14:03:42Z
dc.date2015-11-26T17:40:39Z
dc.date2014-07-30T14:03:42Z
dc.date2015-11-26T17:40:39Z
dc.date.accessioned2018-03-29T00:22:21Z
dc.date.available2018-03-29T00:22:21Z
dc.identifierArchives Of Virology. Springer-verlag Wien, v. 145, n. 5, n. 1009, n. 1019, 2000.
dc.identifier0304-8608
dc.identifierWOS:000087517700013
dc.identifier10.1007/s007050050691
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/57775
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/57775
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1286787
dc.descriptionA previously uncharacterized virus was reported in southeast Brazil causing a yellowing leaf disease in sugarcane. The virus, termed sugarcane yellow leaf virus (ScYLV), shares features typical of the luteoviruses. To start the molecular characterization of ScYLV, the nucleotide sequence of the coat protein (CP), 17 kDa protein and C-tenminus of the RNA-dependent RNA polymerase coding regions was determined from an RT-PCR amplification product. Comparisons showed that the deduced amino acid sequences share a considerable degree of identity and similarity with corresponding sequences of known luteoviruses, thus clearly establishing ScYLV as a member of the family Luteoviridae. The authenticity of the CP open reading frame was confirmed by its expression in Escherichia coli. The recombinant CP positively reacted in immunoblot assays with polyclonal antibodies raised against native ScYLV. Furthermore, phylogenetic analyses also suggest that the 5' and 3' coding blocks of the ScYLV genome possess different taxonomic affinities within the Luteoviridae family, as does also the genome of soybean dwarf virus.
dc.description145
dc.description5
dc.description1009
dc.description1019
dc.languageen
dc.publisherSpringer-verlag Wien
dc.publisherVienna
dc.publisherAustria
dc.relationArchives Of Virology
dc.relationArch. Virol.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectNucleotide-sequence
dc.subjectDwarf Virus
dc.subjectGenomic Rna
dc.subjectOrganization
dc.subjectPolymerase
dc.titleMolecular evidence that sugarcane yellow leaf virus (ScYLV) is a member of the Luteoviridae family
dc.typeArtículos de revistas


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