New techniques for the recovery of small amounts of mature enamel proteins

dc.creatorPorto, IM
dc.creatorLaure, HJ
dc.creatorde Sousa, FB
dc.creatorRosa, JC
dc.creatorGerlach, RF
dc.date2011
dc.dateDEC
dc.date2014-07-30T14:48:20Z
dc.date2015-11-26T17:40:33Z
dc.date2014-07-30T14:48:20Z
dc.date2015-11-26T17:40:33Z
dc.date.accessioned2018-03-29T00:22:16Z
dc.date.available2018-03-29T00:22:16Z
dc.identifierJournal Of Archaeological Science. Academic Press Ltd- Elsevier Science Ltd, v. 38, n. 12, n. 3596, n. 3604, 2011.
dc.identifier0305-4403
dc.identifierWOS:000297384300042
dc.identifier10.1016/j.jas.2011.08.030
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/61900
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/61900
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1286762
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCurrently there are no non-destructive techniques to obtain protein from the dental enamel, the most mineralized tissue in mammals and most resistant to diagenesis, which provides a window to the developing period by means of incremental markings containing proteins. To recover protein, dissolution of powdered enamel is required. Here we tested whether samples obtained by micro-etching of the enamel surface were adequate for protein analysis by MALDI-TOF/TOF mass spectrometry and identification in protein databases. The micro-etch techniques were effective in generating adequate samples for mass spectrometry (from 3 to 13.4 mu m superficial enamel), being also highly conservative, since they rendered masses of enamel ranging from 0.1 to 0.4 mg. Using these techniques the separation of proteins by SDS-PAGE was not necessary, and the whole procedure was easier. Results showed successful identification of specific enamel proteins after whole crown superficial etching with 11% EDTA in the case of immature porcine samples, and with 10% HCl in the case of mature human enamel. X- and Y-isoforms of amelogenin, ameloblastin, and enamelin peptides were identified. The new techniques described here allowed the successful recovery of enamel proteins, opening new avenues for the use of enamel protein information in fossil/archeological material, where sometimes little protein is left. (C) 2011 Elsevier Ltd. All rights reserved.
dc.description38
dc.description12
dc.description3596
dc.description3604
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageen
dc.publisherAcademic Press Ltd- Elsevier Science Ltd
dc.publisherLondon
dc.publisherInglaterra
dc.relationJournal Of Archaeological Science
dc.relationJ. Archaeol. Sci.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectEnamel
dc.subjectMALDI-TOF
dc.subjectProtein extraction
dc.subjectEnamel proteins
dc.subjectAmelogenin
dc.subjectDental Enamel
dc.subjectIn-vivo
dc.subjectLead
dc.subjectChildren
dc.subjectTeeth
dc.subjectExtraction
dc.subjectSequence
dc.subjectGene
dc.titleNew techniques for the recovery of small amounts of mature enamel proteins
dc.typeArtículos de revistas


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