Artículos de revistas
Differential sRNA Regulation in Leaves and Roots of Sugarcane under Water Depletion
Registro en:
Plos One. Public Library Science, v. 9, n. 4, 2014.
1932-6203
WOS:000334103000126
10.1371/journal.pone.0093822
Autor
Thiebaut, F
Grativol, C
Tanurdzic, M
Carnavale-Bottino, M
Vieira, T
Motta, MR
Rojas, C
Vincentini, R
Chabregas, SM
Hemerly, AS
Martienssen, RA
Ferreira, PCG
Institución
Resumen
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Plants have developed multiple regulatory mechanisms to respond and adapt to stress. Drought stress is one of the major constraints to agricultural productivity worldwide and recent reports have highlighted the importance of plant sRNA in the response and adaptation to water availability. In order to increase our understanding of the roles of sRNA in response to water depletion, cultivars of sugarcane were submitted to treatment of ceasing drip irrigation for 24 hours. Deep sequencing analysis was carried out to identify the sRNA regulated in leaves and roots of sugarcane cultivars with different drought sensitivities. The pool of sRNA selected allowed the analysis of different sRNA classes ( miRNA and siRNA). Twenty-eight and 36 families of conserved miRNA were identified in leaf and root libraries, respectively. Dynamic regulation of miRNA was observed and the expression profiles of eight miRNA were verified in leaf samples from three biological replicates by stem-loop qRT-PCR assay using the cultivars: SP90-1638-sensitive cultivar- and; SP83-2847 and SP83-5073 tolerant cultivars. Altered miRNA regulation was correlated with changes in mRNA levels of specific targets. Two leaf libraries from individual sugarcane cultivars with contrasting drought-tolerance properties were also analyzed. An enrichment of 22-nt sRNA species was observed in leaf libraries. 22-nt miRNA triggered siRNA production by cleavage of their targets in response to water depletion. A number of genes of the sRNA biogenesis pathway were down-regulated in tolerant genotypes and up-regulated in sensitive in response to water depletion treatment. Our analysis contributes to increase the knowledge on the roles of sRNA in sugarcane submitted to water depletion. 9 4 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Instituto Nacional de Ciencia e Tecnologia em Fixacao Biologica de Nitrogenio (INCT) Financiadora de Estudos e Projetos (FINEP) Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)