Mathematical modeling and simulation of inulinase adsorption in expanded bed column

dc.creatorMoraes, CC
dc.creatorMazutti, MA
dc.creatorRodrigues, MI
dc.creatorMaugeri, F
dc.creatorKalil, SJ
dc.date2009
dc.dateMAY 15
dc.date2014-11-17T15:50:12Z
dc.date2015-11-26T17:40:02Z
dc.date2014-11-17T15:50:12Z
dc.date2015-11-26T17:40:02Z
dc.date.accessioned2018-03-29T00:21:41Z
dc.date.available2018-03-29T00:21:41Z
dc.identifierJournal Of Chromatography A. Elsevier Science Bv, v. 1216, n. 20, n. 4395, n. 4401, 2009.
dc.identifier0021-9673
dc.identifierWOS:000265956100012
dc.identifier10.1016/j.chroma.2009.03.027
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/53320
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/53320
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/53320
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1286615
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionA mathematical model for an expanded bed column was developed to predict breakthrough curves for inulinase adsorption on Streamline SP ion-exchange adsorbent, using a crude fermentative broth with cells as the feedstock. The kinetics and mass transfer parameters were estimated using the PSO (particle swarm optimization) heuristic algorithm. The parameters were estimated for each expansion degree (ED) using three breakthrough curves at initial inulinase concentrations of 65.6U mL(-1). In sequence, the model parameters for an ED of 2.5 were validated using the breakthrough curve at an initial concentration of 114.4 U mL(-1). The applicability of the validated model in process optimization was investigated, using the model as a process simulator and experimental design methodology to optimize the column and process efficiencies. The results demonstrated the usefulness of this methodology for expanded bed adsorption processes. (C) 2009 Elsevier B.V. All rights reserved.
dc.description1216
dc.description20
dc.description4395
dc.description4401
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationJournal Of Chromatography A
dc.relationJ. Chromatogr. A
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectInulinase
dc.subjectExpanded bed
dc.subjectMathematical modeling
dc.subjectParameter estimation
dc.subjectExperimental design
dc.subjectEBA optimization
dc.subjectExchange Chromatography Step
dc.subjectKluyveromyces-marxianus
dc.subjectProtein Adsorption
dc.subjectRobustness Analysis
dc.subjectOptimization
dc.subjectPurification
dc.subjectBreakthrough
dc.subjectAdsorbent
dc.titleMathematical modeling and simulation of inulinase adsorption in expanded bed column
dc.typeArtículos de revistas


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