Enterococcus faecalis in dental root canals detected by culture and by polymerase chain reaction analysis

dc.creatorGomes, BPFA
dc.creatorPinheiro, ET
dc.creatorSousa, ELR
dc.creatorJacinto, RC
dc.creatorZaia, AA
dc.creatorFerraz, CCR
dc.creatorde Souza, FJ
dc.date2006
dc.dateAUG
dc.date2014-11-17T14:46:38Z
dc.date2015-11-26T17:39:34Z
dc.date2014-11-17T14:46:38Z
dc.date2015-11-26T17:39:34Z
dc.date.accessioned2018-03-29T00:21:08Z
dc.date.available2018-03-29T00:21:08Z
dc.identifierOral Surgery Oral Medicine Oral Pathology Oral Radiology And Endodontics. Mosby, Inc, v. 102, n. 2, n. 247, n. 253, 2006.
dc.identifier1079-2104
dc.identifierWOS:000239667800025
dc.identifier10.1016/j.tripleo.2005.11.031
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/64889
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/64889
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/64889
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1286481
dc.descriptionObjective. The objective of this study was to investigate the presence of Enterococcus faecalis in endodontic infections by culture and polymerase chain reaction analyses. Study design. Microbial samples were obtained from 50 teeth with untreated necrotic pulps (primary infection) and from 50 teeth with failing encloclontic treatment (secondary infection). Culture techniques were used including serial dilution, plating, incubation, and biochemical identification. For PCR detection, samples were analyzed using a species-specific primer of the 16S rDNA and the downstream intergenic spacer region. Results. Culture and PCR detected the test species in 23 of 100 and 79 of 100 of the teeth, respectively. E faecalis was cultured from 2 (4%) of 50 necrotic canals and from 21 (42%) of 50 root-treated canals. PCR detection identified the target species in 41 (82%) and 38 (76%) of 50 primary and secondary infections respectively. Conclusion. E faecalis was detected as frequently in teeth with necrotic pulp as in teeth with failing endodontic treatment when a PCR analysis was used.
dc.description102
dc.description2
dc.description247
dc.description253
dc.languageen
dc.publisherMosby, Inc
dc.publisherSt Louis
dc.publisherEUA
dc.relationOral Surgery Oral Medicine Oral Pathology Oral Radiology And Endodontics
dc.relationOral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectFailed Endodontic Treatment
dc.subjectFilled Teeth
dc.subjectPorphyromonas-gingivalis
dc.subjectStrain Identification
dc.subjectApical Periodontitis
dc.subjectCalcium Hydroxide
dc.subjectHuman Mouth
dc.subjectMicroorganisms
dc.subjectBacteria
dc.subjectInfections
dc.titleEnterococcus faecalis in dental root canals detected by culture and by polymerase chain reaction analysis
dc.typeArtículos de revistas


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