Insulin induces tyrosine phosphorylation of Shc and stimulates Shc/GRB2 association in insulin-sensitive tissues of the intact rat

dc.creatorPaez-Espinosa, V
dc.creatorCarvalho, CRO
dc.creatorAlvarez-Rojas, F
dc.creatorJaneri, L
dc.creatorVelloso, LA
dc.creatorBoschero, AC
dc.creatorSaad, MJA
dc.date1998
dc.dateAPR
dc.date2014-12-02T16:30:11Z
dc.date2015-11-26T17:39:09Z
dc.date2014-12-02T16:30:11Z
dc.date2015-11-26T17:39:09Z
dc.date.accessioned2018-03-29T00:20:45Z
dc.date.available2018-03-29T00:20:45Z
dc.identifierEndocrine. Humana Press Inc, v. 8, n. 2, n. 193, n. 200, 1998.
dc.identifier0969-711X
dc.identifierWOS:000074392400012
dc.identifier10.1385/ENDO:8:2:193
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/60295
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/60295
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/60295
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1286382
dc.descriptionShc is a novel type of tyrosine-phosphorylated protein activated in response to a wide variety of polypeptide ligands. In this study, we used immunoprecipitation and immunoblotting to examine the effect of insulin on Shc tyrosine phosphorylation and Shc/GRB2 association in insulin-sensitive tissues of the intact rat. Following an infusion of insulin, Shc was tyrosine-phosphorylated in the liver, skeletal muscle, and adipose tissue in a time- and dose-dependent fashion, which peaked 5 min after exposure to the hormone and, except in the case of adipose tissue, returned to basal values after 15 min. There was coimmunoprecipitation of Shc and the insulin receptor after stimulation with insulin. Receptor tyrosine kinase activity toward Shc was also observed. Following an infusion of insulin, Shc was found to associate with GRB2. These results demonstrate that after stimulation of rat tissues with insulin, Shc binds to the insulin receptor, is tyrosine-phosphorylated, and subsequently associates with GRB2.
dc.description8
dc.description2
dc.description193
dc.description200
dc.languageen
dc.publisherHumana Press Inc
dc.publisherTotowa
dc.publisherEUA
dc.relationEndocrine
dc.relationEndocrine
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectShc
dc.subjectGRB2
dc.subjecttyrosine phosphorylation
dc.subjecttyrosine kinase activity
dc.subjectGrowth-factor Receptors
dc.subjectPhosphotyrosine-dependent Interaction
dc.subjectFactor-i-receptor
dc.subjectSignal-transduction
dc.subjectP21(ras)-gtp Formation
dc.subjectInteraction Domain
dc.subjectBeta-subunit
dc.subjectNpey Motif
dc.subjectSubstrate-1
dc.subjectProteins
dc.titleInsulin induces tyrosine phosphorylation of Shc and stimulates Shc/GRB2 association in insulin-sensitive tissues of the intact rat
dc.typeArtículos de revistas


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