ARHGAP21 associates with FAK and PKC zeta and is redistributed after cardiac pressure overload

dc.creatorBorges, L
dc.creatorBigarella, CL
dc.creatorBaratti, MO
dc.creatorCrosara-Alberto, DP
dc.creatorJoazeiro, PP
dc.creatorFranchini, KG
dc.creatorCosta, FF
dc.creatorSaad, STO
dc.date2008
dc.dateOCT 3
dc.date2014-07-30T13:50:54Z
dc.date2015-11-26T17:39:00Z
dc.date2014-07-30T13:50:54Z
dc.date2015-11-26T17:39:00Z
dc.date.accessioned2018-03-29T00:20:38Z
dc.date.available2018-03-29T00:20:38Z
dc.identifierBiochemical And Biophysical Research Communications. Academic Press Inc Elsevier Science, v. 374, n. 4, n. 641, n. 646, 2008.
dc.identifier0006-291X
dc.identifierWOS:000258866900009
dc.identifier10.1016/j.bbrc.2008.07.085
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/54880
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/54880
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1286353
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionARHGAP21 is highly expressed in the heart, which demonstrates activity over Cdc42 and interacts with proteins of the cytoskeleton and adherent junctions. The main cause of cardiac hypertrophy is mechanical stimulus; therefore we analyzed ARHGAP21 expression after acute mechanical stress in the myocardium and its association with FAK and PKC zeta. We demonstrated that ARHGAP21 is relocated to Z-lines and costameres after pressure overload, and interacts with PKC zeta and FAK in control rats (sham), rats submitted to aortic clamping and spontaneously hypertensive rats (SHR). Co-transfection using ARHGAP21 and PKC zeta constructions demonstrated that ARHGAP21 associates with PKC zeta-GST and endogenous FAK. Pull-down assay showed that ARHGAP21 binds to the C-terminal region of FAK. Moreover, ARHGAP21 binds to PKC zeta phosphorylated on Thr410 in sham and SHR. However, ARHGAP21 only binds to FAK phosphorylated on Tyr925 of SHR. Additionally, PKC zeta is phosphorylated by mechanical stimuli. These results suggest that ARHGAP21 may act as a signaling or scaffold protein of FAK and PKC zeta signaling pathways, developing an important function during cardiac stress. (c) 2008 Elsevier Inc. All rights reserved.
dc.description374
dc.description4
dc.description641
dc.description646
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageen
dc.publisherAcademic Press Inc Elsevier Science
dc.publisherSan Diego
dc.publisherEUA
dc.relationBiochemical And Biophysical Research Communications
dc.relationBiochem. Biophys. Res. Commun.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectARHGAP21
dc.subjectcardiomyocyte
dc.subjectacute mechanical stress
dc.subjectrat
dc.subjectcell signaling
dc.subjectFocal Adhesion Kinase
dc.subjectTumor-necrosis-factor
dc.subjectC-zeta
dc.subjectHeart-failure
dc.subjectCell Motility
dc.subjectRat-heart
dc.subjectDifferential Regulation
dc.subjectEarly Activation
dc.subjectProtein
dc.subjectHypertrophy
dc.titleARHGAP21 associates with FAK and PKC zeta and is redistributed after cardiac pressure overload
dc.typeArtículos de revistas


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