Comparative metallomics for transgenic and non-transgenic soybeans

dc.creatorSussulini, A
dc.creatorSouza, GHMF
dc.creatorEberlin, MN
dc.creatorArruda, MAZ
dc.date2007
dc.date2014-11-16T23:43:55Z
dc.date2015-11-26T17:38:36Z
dc.date2014-11-16T23:43:55Z
dc.date2015-11-26T17:38:36Z
dc.date.accessioned2018-03-29T00:20:15Z
dc.date.available2018-03-29T00:20:15Z
dc.identifierJournal Of Analytical Atomic Spectrometry. Royal Soc Chemistry, v. 22, n. 12, n. 1501, n. 1506, 2007.
dc.identifier0267-9477
dc.identifierWOS:000251051500017
dc.identifier10.1039/b706684h
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/56132
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/56132
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/56132
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1286254
dc.descriptionIn this work, a comparative metallomics of transgenic and non- transgenic soybeans [ Glycine max ( L.) Merrill] was performed. Soybean proteins were extracted with a proper buffer and separated by two- dimensional polyacrylamide gel electrophoresis. Metal ions bound to a set of eight proteins randomly selected ( ranging from 13.98 to 54.87 kDa), were characterized by matrixassisted laser desorption- ionization quadrupole- time of flight mass spectrometry and mapped using synchrotron radiation X- ray spectrometry. The metal ions detected were: Ca( II), Cu( II), Fe( II), Mn( II), Ni( II) and Zn( II). Transgenic and non- transgenic soybeans proteins were found to display typical and random profiles for metal ions binding. To test the reliability of the qualitative metal ions profiles, quantification of Ca( II), Cu( II) and Fe( II) was performed via microwave-assisted decomposition in mini- vials followed by atomic absorption spectrometry determination. Qualitative and quantitative metallomics was found to be coherent and to match profiles expected from the known protein functions. The protein of spot 5, with molar mass of 37.62 kDa ( amino acid sequence presented), was found to display the most characteristic change in metal ions content, with higher Ca( II), Cu( II) and Fe( II) concentrations for transgenic soybeans.
dc.description22
dc.description12
dc.description1501
dc.description1506
dc.languageen
dc.publisherRoyal Soc Chemistry
dc.publisherCambridge
dc.publisherInglaterra
dc.relationJournal Of Analytical Atomic Spectrometry
dc.relationJ. Anal. At. Spectrom.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectX-ray-fluorescence
dc.subjectGel-electrophoresis
dc.subjectMetalloprotein Analysis
dc.subjectMass-spectrometry
dc.subjectMetal-binding
dc.subjectMini-vials
dc.subjectProteins
dc.subjectDecomposition
dc.subjectPlants
dc.titleComparative metallomics for transgenic and non-transgenic soybeans
dc.typeArtículos de revistas


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