Cytotoxicity of trans-dehydrocrotonin from Croton cajucara on V79 cells and rat hepatocytes

dc.creatorRodriguez, JA
dc.creatorHaun, M
dc.date1999
dc.dateAUG
dc.date2014-07-30T14:03:26Z
dc.date2015-11-26T17:38:26Z
dc.date2014-07-30T14:03:26Z
dc.date2015-11-26T17:38:26Z
dc.date.accessioned2018-03-29T00:20:04Z
dc.date.available2018-03-29T00:20:04Z
dc.identifierPlanta Medica. Georg Thieme Verlag, v. 65, n. 6, n. 522, n. 526, 1999.
dc.identifier0032-0943
dc.identifierWOS:000082210800005
dc.identifier10.1055/s-1999-14008
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/57648
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/57648
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1286208
dc.descriptionThe cytotoxicity of trans-dehydrocrotonin (DHC), an antiulcerogenic diterpene from Croton cajucara (Euphorbiaceae), was assessed on a V79 fibroblast cell line and on rat hepatocytes. Three independent endpoints for cytotoxicity were evaluated: DNA content, MTT reduction and neutral red uptake (NRU). For the V79 cells IC50 values of 253 and 360 mu M were obtained for the NRU and MTT tests. The cytotoxic effect of DHC was time exposure dependent and no ability to recover after treatment was observed. For the rat hepatocytes IC50 values of 8, 300 and 400 mu M for the MTT, DNA and NRU assays were obtained. The greater toxicity observed for the MTT test was inhibited when the experiment was performed using non-fresh hepatocytes in an age-dependent fashion. The treatment of V79 cells with the conditioned medium resulting after hepatocyte incubation with DHC showed an enhancement of MTT reduction without any evident toxic effects on fibroblasts. These results suggest that DHC has basal cytotoxic effects as observed on V79 fibroblasts and expresses a selective cytotoxicity after its metabolization by the hepatocytes. The bioactivation of DHC is mediated by cytochrome P450 and could generate metabolites that have no toxicity for V79 fibroblasts.
dc.description65
dc.description6
dc.description522
dc.description526
dc.languageen
dc.publisherGeorg Thieme Verlag
dc.publisherStuttgart
dc.publisherAlemanha
dc.relationPlanta Medica
dc.relationPlanta Med.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectCroton cajucara
dc.subjectEuphorbiaceae
dc.subjecttrans-dehydrocrotonin
dc.subjectcytotoxicty
dc.subjectV79 cells
dc.subjecthepatocytes
dc.subjectIn-vitro
dc.subjectCultured-hepatocytes
dc.subjectTetrazolium Dye
dc.subjectMeic Chemicals
dc.subjectToxicity
dc.subjectValidation
dc.subjectAssay
dc.subjectAcetaminophen
dc.subjectDehydrogenase
dc.subjectXenobiotics
dc.titleCytotoxicity of trans-dehydrocrotonin from Croton cajucara on V79 cells and rat hepatocytes
dc.typeArtículos de revistas


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