Production of Cutinase by Fusarium oxysporum on Brazilian Agricultural By-products and its Enantioselective Properties

dc.creatorFraga, LP
dc.creatorCarvalho, PO
dc.creatorMacedo, GA
dc.date2012
dc.dateJAN
dc.date2014-07-30T18:08:29Z
dc.date2015-11-26T17:38:18Z
dc.date2014-07-30T18:08:29Z
dc.date2015-11-26T17:38:18Z
dc.date.accessioned2018-03-29T00:19:55Z
dc.date.available2018-03-29T00:19:55Z
dc.identifierFood And Bioprocess Technology. Springer, v. 5, n. 1, n. 138, n. 146, 2012.
dc.identifier1935-5130
dc.identifierWOS:000299520700015
dc.identifier10.1007/s11947-009-0261-4
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/70403
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/70403
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1286168
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionThe production of cutinase by solid-state fermentation, using by-products such as wheat bran, rice bran, or soybean rind, was carried out using a Fusarium oxysporum strain. The fermentation process was optimized using a central composite design. The best conditions for cutinase production were achieved at 28-30 A degrees C, with water added at 100-150% (w/w) after 72 h of incubation, in the range of 11.7-15.5 U/mL. In addition, the resolution of (R,S)-2-octanol and (R,S)-ibuprofen was performed to evaluate the enantioselectivity of the preparations of cutinase. The cutinase produced from the soybean rind attained higher yields and enantioselectivity in the resolution of (R,S)-2-octanol with octanoic acid in isooctane (E = 9.6). For the (R,S)-ibuprofen resolution, the cutinase produced from rice bran reached the best yields (E = 5.6). This work demonstrated that the enzymes can be produced from different media, such as from by-products or residues rich in carbon sources that do not necessarily present the same biochemical properties, which may be useful for industrial applications.
dc.description5
dc.description1
dc.description138
dc.description146
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageen
dc.publisherSpringer
dc.publisherNew York
dc.publisherEUA
dc.relationFood And Bioprocess Technology
dc.relationFood Bioprocess Technol.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectCutinase
dc.subjectProduction optimization
dc.subjectEnantioselectivity
dc.subjectFusarium oxysporum
dc.subjectSolid-state Fermentation
dc.subjectSecondary Metabolite Production
dc.subjectSolani-pisi
dc.subjectRacemic Ibuprofen
dc.subjectCeratocystis-ulmi
dc.subjectEnzyme-production
dc.subjectLipases
dc.subjectTemperature
dc.subjectDimorphism
dc.subjectAdvantages
dc.titleProduction of Cutinase by Fusarium oxysporum on Brazilian Agricultural By-products and its Enantioselective Properties
dc.typeArtículos de revistas


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