Evaluation of Amino Acid O-Phosphoserine as Ligand for the Capture of Immunoglubulin G from Human Serum

dc.creatorBresolin, ITL
dc.creatorBueno, SMA
dc.date2012
dc.dateJUN
dc.date2014-07-30T17:27:52Z
dc.date2015-11-26T17:37:57Z
dc.date2014-07-30T17:27:52Z
dc.date2015-11-26T17:37:57Z
dc.date.accessioned2018-03-29T00:19:37Z
dc.date.available2018-03-29T00:19:37Z
dc.identifierApplied Biochemistry And Biotechnology. Humana Press Inc, v. 167, n. 3, n. 632, n. 644, 2012.
dc.identifier0273-2289
dc.identifierWOS:000304904200018
dc.identifier10.1007/s12010-012-9679-7
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/65752
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/65752
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1286092
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThe amino acid ortho-phosphoserine (OPS) immobilized on agarose gel was evaluated as a ligand for adsorption of polyclonal human immunoglobulin G (IgG) from human serum in the presence of low ionic strength buffers. Screening of buffer systems showed sodium phosphate as the buffer that exhibited higher IgG purity values. Through breakthrough curve analysis for agarose-OPS (feeding of 31.93 mg of total protein per mL of gel), a purification factor of 5.4 with an IgG purity of 89 % was obtained (based on IgG, IgM, IgA, HSA, and Trf nephelometric analysis). IgG adsorption equilibrium studies showed that these data followed the Langmuir-Freundlich model, with cooperativity parameter (n) equal to 1.74, indicating the presence of positive cooperativity, probably due to multipoint interactions. The maximum IgG binding capacity was 24.2 mg mL(-1), near the value for the bioaffinity ligand protein A. The agarose-OPS adsorbent provides an attractive alternative for capturing of IgG from human serum.
dc.description167
dc.description3
dc.description632
dc.description644
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageen
dc.publisherHumana Press Inc
dc.publisherTotowa
dc.publisherEUA
dc.relationApplied Biochemistry And Biotechnology
dc.relationAppl. Biochem. Biotechnol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectO-phosphoserine
dc.subjectAmino acid
dc.subjectHuman immunoglobulin G
dc.subjectAdsorption
dc.subjectPurification
dc.subjectHuman serum
dc.subjectHollow-fiber Membranes
dc.subjectHuman-immunoglobulin-g
dc.subjectAffinity-chromatography
dc.subjectHuman Plasma
dc.subjectPseudobioaffinity Chromatography
dc.subjectGamma-globulin
dc.subjectBuffer Systems
dc.subjectL-histidine
dc.subjectHuman-igg
dc.subjectPurification
dc.titleEvaluation of Amino Acid O-Phosphoserine as Ligand for the Capture of Immunoglubulin G from Human Serum
dc.typeArtículos de revistas


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