Thermal and photochemical nitric oxide release from S-nitrosothiols incorporated in pluronic F127 gel: potential uses for local and controlled nitric oxide release

dc.creatorShishido, SM
dc.creatorSeabra, AB
dc.creatorLoh, W
dc.creatorde Oliveira, MG
dc.date2003
dc.dateSEP
dc.date2014-11-17T11:51:51Z
dc.date2015-11-26T17:37:21Z
dc.date2014-11-17T11:51:51Z
dc.date2015-11-26T17:37:21Z
dc.date.accessioned2018-03-29T00:19:01Z
dc.date.available2018-03-29T00:19:01Z
dc.identifierBiomaterials. Elsevier Sci Ltd, v. 24, n. 20, n. 3543, n. 3553, 2003.
dc.identifier0142-9612
dc.identifierWOS:000183645100019
dc.identifier10.1016/S0142-9612(03)00153-4
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/57980
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/57980
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/57980
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1285940
dc.descriptionThe local delivery of nitric oxide (nitrogen monoxide, NO) by thermal or photochemical means to target cells or organs has a great potential in several biomedical applications, especially if the NO donors are incorporated into non-toxic viscous matrices. In this work, we have shown that the NO donors S-nitrosoglutathione (GSNO) and S-nitroso-N-acetylcysteine (SNAC) can be incorporated into F 127 hydrogels, from where NO can be released thermally or photochemically (with lambda(irr) > 480 nm). High sensitivity differential scanning calorimetry (HSDSC) and a new spectrophotometric method, were used to characterize the micellization and the reversal thermal gelation processes of the F 127 hydrogels containing NO donors, and to modulate the gelation temperatures to the range 29-32degreesC. Spectral monitoring of the S-NO bond cleavage showed that the initial rates of thermal and photochemical NO release (ranging from 2 to 45 mumol 1(-1) min(-1)) are decreased in the hydrogel matrices, relative to those obtained in aqueous solutions. This stabilization effect was assigned to a cage recombination mechanism and offers an additional advantage for the storage and handling of S-nitrosothiols. These results indicate that F127 hydrogels might be used for the thermal and photochemical delivery of NO from S-nitrosothiols to target areas in biomedical applications. (C) 2003 Elsevier Science Ltd. All rights reserved.
dc.description24
dc.description20
dc.description3543
dc.description3553
dc.languageen
dc.publisherElsevier Sci Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationBiomaterials
dc.relationBiomaterials
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectnitric oxide release
dc.subjectS-nitrosothiols
dc.subjectPEO-PPO-PEO copolymers
dc.subjecthydrogels
dc.subjectphotolysis
dc.subjectBlock-copolymers
dc.subjectF-127 Gels
dc.subjectPlatelet-adhesion
dc.subjectAqueous-solution
dc.subjectL-arginine
dc.subjectAggregation Behavior
dc.subjectTriblock Copolymers
dc.subjectMicellar Solutions
dc.subjectNitrosoglutathione
dc.subjectMechanism
dc.titleThermal and photochemical nitric oxide release from S-nitrosothiols incorporated in pluronic F127 gel: potential uses for local and controlled nitric oxide release
dc.typeArtículos de revistas


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