dc.creator | Freire, MDM | |
dc.creator | Machado, OLT | |
dc.creator | Smolka, MB | |
dc.creator | Marangoni, S | |
dc.creator | Novello, JC | |
dc.creator | Macedo, MLR | |
dc.date | 2001 | |
dc.date | AUG | |
dc.date | 2014-11-17T11:47:57Z | |
dc.date | 2015-11-26T17:37:17Z | |
dc.date | 2014-11-17T11:47:57Z | |
dc.date | 2015-11-26T17:37:17Z | |
dc.date.accessioned | 2018-03-29T00:18:57Z | |
dc.date.available | 2018-03-29T00:18:57Z | |
dc.identifier | Journal Of Protein Chemistry. Kluwer Academic/plenum Publ, v. 20, n. 6, n. 495, n. 500, 2001. | |
dc.identifier | 0277-8033 | |
dc.identifier | WOS:000172269800007 | |
dc.identifier | 10.1023/A:1012510614429 | |
dc.identifier | http://www.repositorio.unicamp.br/jspui/handle/REPOSIP/60812 | |
dc.identifier | http://www.repositorio.unicamp.br/handle/REPOSIP/60812 | |
dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/60812 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1285918 | |
dc.description | Four isolectins (TEL-I, TEL-II, TEL-III and TEL-IV ) were isolated from seeds of Talisia esculenta by reverse-phase high-performance liquid chromatography. RP-HPLC was performed on a u-Bondapack C18 column (0.78 cm x 30 cm) (Waters 991-PDA system) at room temperature. Rechromatography of the four fractions on a C18 column under the same conditions yielded lectins with two dissimilar subunits (M-r 20 kDa and 40 kDa) bound noncovalently. The isolectins showed very similar characteristics, such as molecular masses, N-terminal sequences, and hemagglutinating activity, but differed in their isoelectric points and in inhibition by carbohydrates. | |
dc.description | 20 | |
dc.description | 6 | |
dc.description | 495 | |
dc.description | 500 | |
dc.language | en | |
dc.publisher | Kluwer Academic/plenum Publ | |
dc.publisher | New York | |
dc.publisher | EUA | |
dc.relation | Journal Of Protein Chemistry | |
dc.relation | J. Protein Chem. | |
dc.rights | fechado | |
dc.source | Web of Science | |
dc.subject | isolectin | |
dc.subject | reverse-phase HPLC | |
dc.subject | Talisia esculenta seeds | |
dc.subject | Purification | |
dc.subject | Lectins | |
dc.subject | Proteins | |
dc.subject | Complex | |
dc.title | Isolation and characterization of isolectins from Talisia esculenta seeds | |
dc.type | Artículos de revistas | |