Feulgen-DNA response and chromatin condensation in Malpighian tubules of Melipona rufiventris and Melipona quadrifasciata (Hymenoptera, Apoidea)

dc.creatorMampumbu, AR
dc.creatorMello, MLS
dc.date2008
dc.dateAUG
dc.date2014-07-30T17:34:51Z
dc.date2015-11-26T17:37:14Z
dc.date2014-07-30T17:34:51Z
dc.date2015-11-26T17:37:14Z
dc.date.accessioned2018-03-29T00:18:54Z
dc.date.available2018-03-29T00:18:54Z
dc.identifierCell Biology International. Academic Press Ltd Elsevier Science Ltd, v. 32, n. 8, n. 935, n. 939, 2008.
dc.identifier1065-6995
dc.identifierWOS:000260671600010
dc.identifier10.1016/j.cellbi.2008.04.010
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/66894
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/66894
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1285906
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionMelipona quadrifasciata and Melipona rufiventris are stingless bee species which present low and high heterochromatin content, respectively, on their mitotic chromosomes as assessed visually after a C-banding assay. However, these species do not show differences in the C-banding responses of their Malpighian tubule interphase nuclei. In the present study, the Feulgen-DNA response, which could inform on differences in DNA depurination due to differences in chromatin condensation, was compared in the cell nuclei of the Malpighian tubules of these species. It was hypothesized that differences in acid hydrolysis kinetics patterns, as assessed by Feulgen reaction and studied microspectrophotometrically, could discriminate M. quadrifasciata and M. rufiventris interphase nuclei not distinguishable with the C-banding method. Feulgen-DNA values corresponding to more than one ploidy class were found in both species; these values at the hydrolysis time corresponding to the maximal DNA depurination for each ploidy degree were higher in M. quadrifasciata, reflecting a higher DNA content in the Malpighian tubule cell nuclei of this species compared to those of M. rufiventris at the same larval instar. The maximal Feulgen-DNA values of M. quadrifasciata after short (50 min) and long (90 min) hydrolysis times were found to be closer to each other, while those of M. rufiventris occurred sharply at the long hydrolysis time, indicating that DNA depurination in M. quadrifasciata occurred faster. This result is probably related to the involvement of differences in chromatin condensation; it agrees with the idea that M. rufiventris contains more heterochromatin than M. quadrifasciata, which is supported by the analysis of results obtained with the image analysis parameter average absorption ratio. The depurination kinetics studied here with the Feulgen reaction were revealed to be more pertinent than the C-banding technique in establishing differences in levels of chromatin condensation for these cell nuclei. (c) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
dc.description32
dc.description8
dc.description935
dc.description939
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageen
dc.publisherAcademic Press Ltd Elsevier Science Ltd
dc.publisherLondon
dc.publisherInglaterra
dc.relationCell Biology International
dc.relationCell Biol. Int.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectChromatin condensation
dc.subjectFeulgen-DNA
dc.subjectHeterochromatin
dc.subjectMalpighian tubules
dc.subjectMelipona
dc.subjectCell-death
dc.subjectHeterochromatin
dc.subjectSupraorganization
dc.subjectFragmentation
dc.subjectErythrocytes
dc.subjectLability
dc.subjectApidae
dc.subjectBands
dc.titleFeulgen-DNA response and chromatin condensation in Malpighian tubules of Melipona rufiventris and Melipona quadrifasciata (Hymenoptera, Apoidea)
dc.typeArtículos de revistas


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