dc.creator | Mello, MLS | |
dc.creator | Russo, P | |
dc.creator | Russo, J | |
dc.creator | Vidal, BC | |
dc.date | 2007 | |
dc.date | DEC | |
dc.date | 2014-11-16T01:29:04Z | |
dc.date | 2015-11-26T17:37:05Z | |
dc.date | 2014-11-16T01:29:04Z | |
dc.date | 2015-11-26T17:37:05Z | |
dc.date.accessioned | 2018-03-29T00:18:45Z | |
dc.date.available | 2018-03-29T00:18:45Z | |
dc.identifier | Oncology Reports. Professor D A Spandidos, v. 18, n. 6, n. 1475, n. 1481, 2007. | |
dc.identifier | 1021-335X | |
dc.identifier | WOS:000251353300018 | |
dc.identifier | http://www.repositorio.unicamp.br/jspui/handle/REPOSIP/52525 | |
dc.identifier | http://www.repositorio.unicamp.br/handle/REPOSIP/52525 | |
dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/52525 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1285869 | |
dc.description | Treatment of the human breast epithelial cells MCF-10F with 17-beta-estradiol (E2) induces transformation and tumorigenesis. E2-transformed MCF-10F cells are known to exhibit progressive loss of ductulogenesis, and invasive and tumorigenic phenotypes. Although DNA amounts and chromatin supraorganization change in E2-transformed MCF cells, no comparative study has yet been undertaken in the resulting cells selected for aggressive invasiveness (C5) and tumor generation in a heterologous host. The aim of this study was thus to determine whether changes in Feulgen-DNA content and chromatin supraorganization are involved during E2-induced transformation and tumorigenesis of the MCF-10F cells. Image analysis was performed for nontransformed and E2-transformed MCF cells, highly invasive cells (C5), and for cell lines (C5-A6-T6 and C5-A8-T8) derived from tumors generated by injection of C5 cells in SCID mice. A decrease in Feulgen-DNA amounts and nuclear sizes induced by E2 treatment was accented with selection of the highly invasive tumorigenesis potential. However, in the tumor-derived cells a high variability in cellular phenotypes resulted inclusively in near-polyploidy. Significant changes in textural parameters, including nuclear entropy, indicated chromatin structural remodeling with advancing tumorigenesis. An increased variability in the degree of chromatin packing states in the E2-transformed MCF cells is followed by reduction in chromatin condensation and in contrast between condensed and noncondensed chromatin in the highly invasive C5 cells and tumor-derived cell lines. Studies on epigenetic mechanisms involving DNA methylation and/or the histone code would contribute to a better interpretation of the chromatin supraorganization changes reported herein. | |
dc.description | O TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE FEVEREIRO DE 2015. | |
dc.description | 18 | |
dc.description | 6 | |
dc.description | 1475 | |
dc.description | 1481 | |
dc.language | en | |
dc.publisher | Professor D A Spandidos | |
dc.publisher | Athens | |
dc.publisher | Grécia | |
dc.relation | Oncology Reports | |
dc.relation | Oncol. Rep. | |
dc.rights | embargo | |
dc.source | Web of Science | |
dc.subject | human breast epithelial cells | |
dc.subject | 17-beta-estradiol | |
dc.subject | tumor-derived cells | |
dc.subject | chromatin packing states | |
dc.subject | image analysis | |
dc.subject | Comparative Genomic Hybridization | |
dc.subject | Tumor-suppressor Gene | |
dc.subject | Dna-content | |
dc.subject | Chromatin Texture | |
dc.subject | Chemical Carcinogens | |
dc.subject | Binding-proteins | |
dc.subject | Cancer Cells | |
dc.subject | Estrogen | |
dc.subject | Transformation | |
dc.subject | Expression | |
dc.title | 17-beta-estradiol affects nuclear image properties in MCF-10F human breast epithelial cells with tumorigenesis | |
dc.type | Artículos de revistas | |