17-beta-estradiol affects nuclear image properties in MCF-10F human breast epithelial cells with tumorigenesis

dc.creatorMello, MLS
dc.creatorRusso, P
dc.creatorRusso, J
dc.creatorVidal, BC
dc.date2007
dc.dateDEC
dc.date2014-11-16T01:29:04Z
dc.date2015-11-26T17:37:05Z
dc.date2014-11-16T01:29:04Z
dc.date2015-11-26T17:37:05Z
dc.date.accessioned2018-03-29T00:18:45Z
dc.date.available2018-03-29T00:18:45Z
dc.identifierOncology Reports. Professor D A Spandidos, v. 18, n. 6, n. 1475, n. 1481, 2007.
dc.identifier1021-335X
dc.identifierWOS:000251353300018
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/52525
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/52525
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/52525
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1285869
dc.descriptionTreatment of the human breast epithelial cells MCF-10F with 17-beta-estradiol (E2) induces transformation and tumorigenesis. E2-transformed MCF-10F cells are known to exhibit progressive loss of ductulogenesis, and invasive and tumorigenic phenotypes. Although DNA amounts and chromatin supraorganization change in E2-transformed MCF cells, no comparative study has yet been undertaken in the resulting cells selected for aggressive invasiveness (C5) and tumor generation in a heterologous host. The aim of this study was thus to determine whether changes in Feulgen-DNA content and chromatin supraorganization are involved during E2-induced transformation and tumorigenesis of the MCF-10F cells. Image analysis was performed for nontransformed and E2-transformed MCF cells, highly invasive cells (C5), and for cell lines (C5-A6-T6 and C5-A8-T8) derived from tumors generated by injection of C5 cells in SCID mice. A decrease in Feulgen-DNA amounts and nuclear sizes induced by E2 treatment was accented with selection of the highly invasive tumorigenesis potential. However, in the tumor-derived cells a high variability in cellular phenotypes resulted inclusively in near-polyploidy. Significant changes in textural parameters, including nuclear entropy, indicated chromatin structural remodeling with advancing tumorigenesis. An increased variability in the degree of chromatin packing states in the E2-transformed MCF cells is followed by reduction in chromatin condensation and in contrast between condensed and noncondensed chromatin in the highly invasive C5 cells and tumor-derived cell lines. Studies on epigenetic mechanisms involving DNA methylation and/or the histone code would contribute to a better interpretation of the chromatin supraorganization changes reported herein.
dc.descriptionO TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE FEVEREIRO DE 2015.
dc.description18
dc.description6
dc.description1475
dc.description1481
dc.languageen
dc.publisherProfessor D A Spandidos
dc.publisherAthens
dc.publisherGrécia
dc.relationOncology Reports
dc.relationOncol. Rep.
dc.rightsembargo
dc.sourceWeb of Science
dc.subjecthuman breast epithelial cells
dc.subject17-beta-estradiol
dc.subjecttumor-derived cells
dc.subjectchromatin packing states
dc.subjectimage analysis
dc.subjectComparative Genomic Hybridization
dc.subjectTumor-suppressor Gene
dc.subjectDna-content
dc.subjectChromatin Texture
dc.subjectChemical Carcinogens
dc.subjectBinding-proteins
dc.subjectCancer Cells
dc.subjectEstrogen
dc.subjectTransformation
dc.subjectExpression
dc.title17-beta-estradiol affects nuclear image properties in MCF-10F human breast epithelial cells with tumorigenesis
dc.typeArtículos de revistas


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