Biological and biochemical characterization of two new PLA(2) isoforms Cdc-9 and Cdc-10 from Crotalus durissus cumanensis snake venom

dc.creatorRomero-Vargas, FF
dc.creatorPonce-Soto, LA
dc.creatorMartins-de-Souza, D
dc.creatorMarangoni, S
dc.date2010
dc.dateJAN
dc.date2014-11-16T00:57:34Z
dc.date2015-11-26T17:37:03Z
dc.date2014-11-16T00:57:34Z
dc.date2015-11-26T17:37:03Z
dc.date.accessioned2018-03-29T00:18:44Z
dc.date.available2018-03-29T00:18:44Z
dc.identifierComparative Biochemistry And Physiology C-toxicology & Pharmacology. Elsevier Science Inc, v. 151, n. 1, n. 66, n. 74, 2010.
dc.identifier1532-0456
dc.identifierWOS:000273152400008
dc.identifier10.1016/j.cbpc.2009.08.011
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55591
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/55591
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55591
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1285864
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionThis work reports the purification, biological characterization and amino acid sequence of two new basic PLA(2) isoforms, Cdc-9 and Cdc-10, purified from the Crotalus durissus cumanensis venom by one step analytical chromatography reverse phase HPLC. The molecular masses of the PLA(2) were 14,175 +/- 2.7 Da for Cdc-9 and 14,228 +/- 3.5 Da for Cdc-10 both deduced by primary structure and confirmed by MALDI-TOF. The isoforms presented an amino acid sequence of 122 amino acid residues, being Cdc-9: SLVQFNKMIK FETRKSGLPF YAAYGCYCGW GGQRPKDATD RCCFVHDCCY GKVAKCNTKW DIYSYSLKSG YITCGKGTWC KEQICECDRV AAECLRRSLS TYKNEYMFYP DSRCREPPEY TC with pI value of 8.25 and Cdc-10: SLLQFNKMIK FETRKSGVPF YAAYGCYCGW GGRRPKDPTD RCCFVHDCCY GKLTKCNTKW DIYSYSLKSG YITCGKGTWC KEQICECDRV AAECLRRSLN TYKNEYMFYP DSRCRGPPEY TC with a pI value of 8.46, showing highly conserved Ca2+-binding and catalytic sites. The PLA(2) activity decreased when the isoforms Cdc-9 and Cdc-10 were incubated with 4-bromophenacyl bromide (p-BPB), anhydrous acetic acid and p-nitrobenzene sulfonyl fluoride (NBSF) when compared with the activity of both native isoforms. In mice, the PLA(2) isoforms Cdc-9 and Cdc-10 induced myonecrosis and edema. Myotoxic and edema activities were reduced after treatment of the isoforms with p-BPB; acetylation of the lysine residues and the treatment of PLA(2) with NBSF have also induced edema reduction. However, p-BPB strongly diminishes the local and systemic myotoxic effects. (C) 2009 Elsevier Inc. All rights reserved.
dc.description151
dc.description1
dc.description66
dc.description74
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageen
dc.publisherElsevier Science Inc
dc.publisherNew York
dc.publisherEUA
dc.relationComparative Biochemistry And Physiology C-toxicology & Pharmacology
dc.relationComp. Biochem. Physiol. C-Toxicol. Pharmacol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectCrotalus durissus cumanensis
dc.subjectRP-HPLC
dc.subjectPLA(2)
dc.subjectMyotoxins
dc.subjectSnake venom
dc.subjectAmino-acid-sequence
dc.subjectSouth-american Rattlesnake
dc.subjectTrimeresurus-flavoviridis Venom
dc.subjectSecreted Phospholipases A(2)
dc.subjectPharmacological-properties
dc.subjectBothrops-asper
dc.subjectLysine Residues
dc.subjectFunctional-characterization
dc.subjectStructural-characterization
dc.subjectEnzymatic Characterization
dc.titleBiological and biochemical characterization of two new PLA(2) isoforms Cdc-9 and Cdc-10 from Crotalus durissus cumanensis snake venom
dc.typeArtículos de revistas


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