Biochemical and biological characterization of a PLA(2) from crotoxin complex of Crotalus durissus cumanensis

dc.creatorPereanez, JA
dc.creatorNunez, V
dc.creatorHuancahuire-Vega, S
dc.creatorMarangoni, S
dc.creatorPonce-Soto, LA
dc.date2009
dc.dateAPR
dc.date2014-11-15T04:35:39Z
dc.date2015-11-26T17:35:58Z
dc.date2014-11-15T04:35:39Z
dc.date2015-11-26T17:35:58Z
dc.date.accessioned2018-03-29T00:18:24Z
dc.date.available2018-03-29T00:18:24Z
dc.identifierToxicon. Pergamon-elsevier Science Ltd, v. 53, n. 5, n. 534, n. 542, 2009.
dc.identifier0041-0101
dc.identifierWOS:000264977700009
dc.identifier10.1016/j.toxicon.2009.01.021
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55504
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/55504
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55504
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1285779
dc.descriptionA new PLA(2) (Cdcum6) from crotoxin complex of Colombian Crotalus durissus cumanensis rattlesnake was purified using molecular exclusion chromatography and RP-HPLC. The molecular mass of Cdcum6 was determined by SDS-PAGE similar to 14 KDa and confirmed by MALDI-TOF (14321.98 Da). The enzyme showed K-m 6.0 mM, V-max 3.44 nmol/min, optimum pH was 8.0 and temperature was between 30 and 45 degrees C, and it had a strict requirement of Ca2+ for its activity. The N-terminal sequence of PLA(2) was SLVQF EKMIK EVAGK NGVPWY. Comparison of amino acid sequence data with other PLA(2) from South American Crotalus durissus rattlesnakes showed that Cdcum6 shares the highest sequence identity with Cdr13 an isoform PLA(2) from Crotalus durissus ruruima, nevertheless, Cdcum6 showed high content of basic and hydrophobic amino acids. In mice, Cdcum6 presented higher LD50 than crotoxin complex from C. d. cumanensis. Additionally, Cdcum6 induced a conspicuous local myotoxic effect and moderate footpad edema; in vitro, it was antigoagulant in doses as low as 0.5 mu g/ml, and it was not cytotoxic on myoblast but Cdcum6 was able to lyse myotubes. (C) 2009 Elsevier Ltd. All rights reserved.
dc.description53
dc.description5
dc.description534
dc.description542
dc.descriptionUniversidad de Antioquia, COLCIENCIAS [393-2006]
dc.descriptionCYTED [206AC0281]
dc.descriptionUniversidad de Antioquia, COLCIENCIAS [393-2006]
dc.descriptionCYTED [206AC0281]
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationToxicon
dc.relationToxicon
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectCrotalus durissus cumanensis
dc.subjectCrotoxin
dc.subjectMyotoxin
dc.subjectNeurotoxin
dc.subjectPhospholipase A(2)
dc.subjectSouth-american Rattlesnake
dc.subjectPhospholipase A(2) Enzymes
dc.subjectAsper Snake-venom
dc.subjectStructural-characterization
dc.subjectTerrificus Venom
dc.subjectBothrops-asper
dc.subjectEnzymatic Characterization
dc.subjectPharmacological Activities
dc.subjectCascavella
dc.subjectIsoforms
dc.titleBiochemical and biological characterization of a PLA(2) from crotoxin complex of Crotalus durissus cumanensis
dc.typeArtículos de revistas


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