Biochemical characterization of heparan sulfate derived from murine hemopoietic stromal cell lines: A bone marrow-derived cell line S17 and a fetal liver-derived cell line AFT024

dc.creatorArcanjo, K
dc.creatorBelo, G
dc.creatorFolco, C
dc.creatorWerneck, CC
dc.creatorBorojevic, R
dc.creatorSilva, LCF
dc.date2002
dc.date2014-11-15T04:22:19Z
dc.date2015-11-26T17:35:58Z
dc.date2014-11-15T04:22:19Z
dc.date2015-11-26T17:35:58Z
dc.date.accessioned2018-03-29T00:18:24Z
dc.date.available2018-03-29T00:18:24Z
dc.identifierJournal Of Cellular Biochemistry. Wiley-liss, v. 87, n. 2, n. 160, n. 172, 2002.
dc.identifier0730-2312
dc.identifierWOS:000178451300005
dc.identifier10.1002/jcb.10293
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55523
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/55523
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55523
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1285778
dc.descriptionHeparan sulfate(HS) present on the surface of hemopoietic stromal cells has important roles in the control of adhesion and growth of hemopoietic stem and progenitor cells. Recent studies have characterized several different heparan sulfate proteoglycans (HSPGs) from both human and murine bone marrow stromal cells. In the present study, we have compared the molecular structure of HS, metabolically labeled with [S-35] -sulfate produced by two distinct preparations of murine hemopoietic stromal cell lines. These comprised a bone marrow-derived cell line S17 and a fetal liver-derived cell line AFT024. [S-35]-HS was examined in the cell layers and in the culture medium. We identified and measured the relative proportions of the various glycosaminoglycans (GAGs) in the two stromal cell lines. Chondroitin sulfate (CS) was preponderantly secreted by the stromal cell lines, while HS was relatively more abundant in the cell-associated fractions. The two types of stromal cells differ in their HS composition, mainly due to different patterns of N- and O-sulfation. The two stromal cell lines expressed mRNA for different HSPGs. Data from reverse transcription PCR revealed that the two stromal cell lines expressed mRNA for glypican and syndecan4. Only AFT024 cell line expressed m RNA for betaglycan. There was no evidence for expression of m RNA for both syndecan1 and syndecan2. [S-35]-sulfated macromolecules could be released from the cell surface of both stromal cell lines by phosphatidylinositol phospholipase C (PI-PLC), which is consistent with the expression of glypican detected by PCR experiments.
dc.description87
dc.description2
dc.description160
dc.description172
dc.languageen
dc.publisherWiley-liss
dc.publisherNew York
dc.publisherEUA
dc.relationJournal Of Cellular Biochemistry
dc.relationJ. Cell. Biochem.
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectglycosaminoglycans
dc.subjectheparan sulfate
dc.subjectglypican
dc.subjectsyndecan
dc.subjecthematopoiesis
dc.subjectColony-stimulating Factor
dc.subjectFibroblast Growth-factor
dc.subjectMessenger-rna
dc.subjectMouse Cdna
dc.subjectIn-vitro
dc.subjectGm-csf
dc.subjectExpression
dc.subjectProteoglycans
dc.subjectBinding
dc.subjectGlycosaminoglycans
dc.titleBiochemical characterization of heparan sulfate derived from murine hemopoietic stromal cell lines: A bone marrow-derived cell line S17 and a fetal liver-derived cell line AFT024
dc.typeArtículos de revistas


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