| dc.creator | Bordeaux-Rego, P | |
| dc.creator | Luzo, A | |
| dc.creator | Costa, FF | |
| dc.creator | Saad, STO | |
| dc.creator | Crosara-Alberto, DP | |
| dc.date | 2010 | |
| dc.date | MAR | |
| dc.date | 2014-11-15T02:35:25Z | |
| dc.date | 2015-11-26T17:35:48Z | |
| dc.date | 2014-11-15T02:35:25Z | |
| dc.date | 2015-11-26T17:35:48Z | |
| dc.date.accessioned | 2018-03-29T00:17:59Z | |
| dc.date.available | 2018-03-29T00:17:59Z | |
| dc.identifier | Stem Cells And Development. Mary Ann Liebert Inc, v. 19, n. 3, n. 413, n. 421, 2010. | |
| dc.identifier | 1547-3287 | |
| dc.identifier | WOS:000275579000014 | |
| dc.identifier | 10.1089/scd.2009.0098 | |
| dc.identifier | http://www.repositorio.unicamp.br/jspui/handle/REPOSIP/78213 | |
| dc.identifier | http://www.repositorio.unicamp.br/handle/REPOSIP/78213 | |
| dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/78213 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1285673 | |
| dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
| dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
| dc.description | Umbilical cord blood (UCB), an ideal source for transplantable hematopoietic stem cells (HSC), is readily available and is rich in progenitor cells. Identification of conditions favoring UCB-HSC ex vivo expansion and of repopulating potential remains a major challenge in hematology. CD133+ cells constitute an earlier, less-differentiated HSC group with a potentially higher engraftment capacity. The presence of SCF, Flt3-L, and TPO are essential for CD133+ and/or CD34+ cells ex vivo expansion; however, IL-3 and IL-6 influence has not yet been clearly established. We investigated this influence on CD133+ cells from UCB ex vivo expansion and the effect of these cytokines upon cell phenotype. Immediately after isolation an 85% of CD133+ cell purity was obtained, diminishing after 4 and 8 days of ex vivo expansion. CD133+ fold-increase was higher using IMDM with SCF, Flt3-L, and TPO (BM)+IL-3 or BM+IL-3+IL-6 on day 8 (13.83- and 17.47-fold increase, respectively). BM+IL-6 presented no significant difference from BM alone. We demonstrated that 5.1% of the CD133+ cells expressed IL-6 receptor (IL-6R) after isolation. After 4 and 8 days in culture, the percentage of CD133+ cells that expressed IL-6R was as follows: BM alone (9.8% and 22.02%, respectively); BM+IL-3 (8.33% and 16.74%); BM+IL-6 (9.2% and 17.67%); and BM+IL-3+IL-6 (12.5% and 61.20%). Cell cycle analysis revealed quiescent cells after isolation, 95.5% CD133+ cells in the G0/G1 phase. Regardless of culture period or cytokine incubation, CD133+ cell cycle altered to 70% of CD133+ in the G0/G1 phase. Colony-forming unit (CFU) doubled in BM+IL-3+IL-6 after 8 days of incubation compared with BM group. SOX-2 and NANOG-relative gene expression was detected on day 0 after isolation. BM+IL-6 prevented the decrease in NANOG and SOX-2 gene expression level compared to BM+IL-3 or BM+IL-3+IL-6 incubated cells. Our results indicated that UCB-isolated CD133+ cells were better ex vivo expanded in the presence of SCF, Flt3-L, TPO, IL-3+IL-6. IL-3 probably promotes higher CD133+ cell expansion and IL-6 maintains immature phenotype. | |
| dc.description | o TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE AGOSTO DE 2015. | |
| dc.description | 19 | |
| dc.description | 3 | |
| dc.description | 413 | |
| dc.description | 421 | |
| dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
| dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
| dc.description | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
| dc.description | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
| dc.language | en | |
| dc.publisher | Mary Ann Liebert Inc | |
| dc.publisher | New Rochelle | |
| dc.publisher | EUA | |
| dc.relation | Stem Cells And Development | |
| dc.relation | Stem Cells Dev. | |
| dc.rights | embargo | |
| dc.source | Web of Science | |
| dc.subject | Hematopoietic Stem-cells | |
| dc.subject | Progenitor Cells | |
| dc.subject | In-vitro | |
| dc.subject | Cd133(+) Cells | |
| dc.subject | Endothelial-cells | |
| dc.subject | Tyrosine Kinase | |
| dc.subject | Self-renewal | |
| dc.subject | Differentiation | |
| dc.subject | Marrow | |
| dc.subject | Expression | |
| dc.title | Both Interleukin-3 and Interleukin-6 Are Necessary for Better Ex Vivo Expansion of CD133+Cells From Umbilical Cord Blood | |
| dc.type | Artículos de revistas | |
