Detection of and phylogenetic studies with avian metapneumovirus recovered from feral pigeons and wild birds in Brazil

dc.creatorFelippe, PA
dc.creatorda Silva, LHA
dc.creatordos Santos, MB
dc.creatorSakata, ST
dc.creatorArns, CW
dc.date2011
dc.date2014-07-30T14:38:54Z
dc.date2015-11-26T17:30:43Z
dc.date2014-07-30T14:38:54Z
dc.date2015-11-26T17:30:43Z
dc.date.accessioned2018-03-29T00:17:35Z
dc.date.available2018-03-29T00:17:35Z
dc.identifierAvian Pathology. Taylor & Francis Ltd, v. 40, n. 5, n. 445, n. 452, 2011.
dc.identifier0307-9457
dc.identifierWOS:000295319700003
dc.identifier10.1080/03079457.2011.596812
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/61288
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/61288
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1285572
dc.descriptionThe aim of the present study was to determine whether avian metapneumovirus (aMPV)-related viruses were present in wild and synanthropic birds in Brazil. Therefore, we analysed samples from wild birds, feral pigeons and domestic chickens in order to perform a phylogenetic comparison. To detect the presence of aMPV, a nested reverse transcriptase-polymerase chain reaction was performed with the aim of amplifying a fragment of 270 bases for subtype A and 330 bases for subtype B, comprising the gene coding the G glycoprotein. Positive samples for aMPV subtypes A and B were found in seven (13.2%) different asymptomatic wild birds and pigeons (50%) that had been received at the Bosque dos Jequitibas Zoo Triage Center, Brazil. Also analysed were positive samples from 15 (12.9%) domestic chickens with swollen head syndrome from several regions of Brazil. The positive samples from wild birds, pigeons and domestic chickens clustered in two major phylogenetic groups: some with aMPV subtype A and others with subtype B. The similarity of the G fragment nucleotide sequence of aMPV isolated from chickens and synanthropic and wild avian species ranged from 100 to 97.5% (from 100 to 92.5% for the amino acids). Some positive aMPV samples, which were obtained from wild birds classified in the Orders Psittaciformes, Anseriformes and Craciformes, clustered with subtype A, and others from the Anas and Dendrocygma genera (Anseriformes Order) with subtype B. The understanding of the epizootiology of aMPV is very important, especially if this involves the participation of non-domestic bird species, which would add complexity to their control on farms and to implementation of vaccination programmes for aMPV.
dc.description40
dc.description5
dc.description445
dc.description452
dc.languageen
dc.publisherTaylor & Francis Ltd
dc.publisherAbingdon
dc.publisherInglaterra
dc.relationAvian Pathology
dc.relationAvian Pathol.
dc.rightsfechado
dc.rightshttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dc.sourceWeb of Science
dc.subjectTurkey Rhinotracheitis Virus
dc.subjectInfectious-bronchitis Virus
dc.subjectPolymerase Chain-reaction
dc.subjectAmino-acid-sequence
dc.subjectMolecular Characterization
dc.subjectNucleotide-sequences
dc.subjectPneumovirus Subtype
dc.subjectCommercial Turkeys
dc.subjectDomestic Turkeys
dc.subjectBroiler Chicken
dc.titleDetection of and phylogenetic studies with avian metapneumovirus recovered from feral pigeons and wild birds in Brazil
dc.typeArtículos de revistas


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