Detection of human herpesvirus-7 by qualitative nested-PCR: comparison between healthy individuals and liver transplant recipients

dc.creatorThomasini, RL
dc.creatorMartins, JD
dc.creatorParola, DC
dc.creatorBonon, SHA
dc.creatorBoin, IDSF
dc.creatorLeonardi, LS
dc.creatorLeonardi, M
dc.creatorCosta, SCB
dc.date2008
dc.dateNOV-DEC
dc.date2014-07-30T14:38:56Z
dc.date2015-11-26T17:29:54Z
dc.date2014-07-30T14:38:56Z
dc.date2015-11-26T17:29:54Z
dc.date.accessioned2018-03-29T00:16:53Z
dc.date.available2018-03-29T00:16:53Z
dc.identifierRevista Da Sociedade Brasileira De Medicina Tropical. Soc Brasileira Medicina Tropical, v. 41, n. 6, n. 556, n. 559, 2008.
dc.identifier0037-8682
dc.identifierWOS:000262440500002
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/61312
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/61312
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1285395
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionDiagnosis of human herpesvirus-7 active infection in transplant patients has proved difficult, because this virus is ubiquitous and can cause persistent infections in the host. The significance of viral DNA detected in leukocytes by PCR is unclear and cross-reaction in serological tests may occur. This study aimed to evaluate nested-PCR to detect human herpesvirus-7 active infection in liver transplant recipients compared to healthy individuals. human herpesvirus-7 nested-PCR was performed on leukocytes and sera of 53 healthy volunteers and sera of 29 liver transplant recipients. In healthy volunteers, human herpesvirus-7 was detected in 28.3% of leukocytes and 0% of serum. human herpesvirus-7 was detected in sera of 48.2% of the liver transplant recipients. Nested-PCR on DNA extracted from leukocytes detected latent infection and the study suggests that nested-PCR performed on serum could be useful to detect human herpesvirus-7 active infection in liver transplant recipients.
dc.description41
dc.description6
dc.description556
dc.description559
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionHerminio Ometto Foundation
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageen
dc.publisherSoc Brasileira Medicina Tropical
dc.publisherBrasilia
dc.publisherBrasil
dc.relationRevista Da Sociedade Brasileira De Medicina Tropical
dc.relationRev. Soc. Bras. Med. Trop.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectHuman herpesvirus-7
dc.subjectHealthy individuals
dc.subjectNested-polymerase chain reaction
dc.subjectLiver transplantation
dc.subjectBlood-donors
dc.subjectCytomegalovirus
dc.subjectInfections
dc.subjectHuman-herpesvirus-7
dc.subjectPrevalence
dc.subjectDisease
dc.titleDetection of human herpesvirus-7 by qualitative nested-PCR: comparison between healthy individuals and liver transplant recipients
dc.typeArtículos de revistas


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