Concentration of alpha-lactalbumin from cow milk whey through expanded bed adsorption using a hydrophobic resin

dc.creatorConrado, LS
dc.creatorVeredas, V
dc.creatorNobrega, ES
dc.creatorSantana, CC
dc.date2005
dc.dateOCT-DEC
dc.date2014-11-17T10:54:45Z
dc.date2015-11-26T17:29:13Z
dc.date2014-11-17T10:54:45Z
dc.date2015-11-26T17:29:13Z
dc.date.accessioned2018-03-29T00:16:14Z
dc.date.available2018-03-29T00:16:14Z
dc.identifierBrazilian Journal Of Chemical Engineering. Brazilian Soc Chemical Eng, v. 22, n. 4, n. 501, n. 509, 2005.
dc.identifier0104-6632
dc.identifierWOS:000231118300002
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/56448
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/56448
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/56448
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1285228
dc.descriptionThe design of novel applications for the use of new high-density adsorbents in the direct recovery of proteins requires analysis of the fluid dynamics and mass transfer characteristics of the column used with the solid-liquid system. In this article we describe the operating parameters related to the use of a high-density, hydrophobic resin (Streamline (R) Phenyl) for the recovery of alpha-lactalbumin from cow milk whey in an expanded-bed adsorption mode of operation. The adsorption isotherm, kinetic curves and chromatographic curves for adsorption and elution were determined for alpha-lactalbumin recovered from whole whey. The experiments were used to confirm the theoretical model that involves the combination of resistance to the mass transfer in the liquid phase and diffusivity in the adsorbent resin pores. The model solution was obtained through the orthogonal collocation method. A nonlinear multivariable optimization method was used to fit the model to experimental results. A purity of 79% was obtained with. the adsorption/elution protocol prior to any polishing procedure.
dc.description22
dc.description4
dc.description501
dc.description509
dc.languageen
dc.publisherBrazilian Soc Chemical Eng
dc.publisherSao Paulo
dc.publisherBrasil
dc.relationBrazilian Journal Of Chemical Engineering
dc.relationBraz. J. Chem. Eng.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectalpha-lactalbumin
dc.subjectmilk whey
dc.subjectexpanded bed adsorption
dc.subjectfluid dynamics
dc.subjectChromatography
dc.titleConcentration of alpha-lactalbumin from cow milk whey through expanded bed adsorption using a hydrophobic resin
dc.typeArtículos de revistas


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