| dc.creator | Almeida, AA | |
| dc.creator | Campos, DR | |
| dc.creator | Bernasconi, G | |
| dc.creator | Calafatti, S | |
| dc.creator | Barros, FAP | |
| dc.creator | Eberlin, MN | |
| dc.creator | Meurer, EC | |
| dc.creator | Paris, EG | |
| dc.creator | Pedrazzoli, J | |
| dc.date | 2007 | |
| dc.date | APR 1 | |
| dc.date | 2014-11-17T12:34:56Z | |
| dc.date | 2015-11-26T17:29:09Z | |
| dc.date | 2014-11-17T12:34:56Z | |
| dc.date | 2015-11-26T17:29:09Z | |
| dc.date.accessioned | 2018-03-29T00:16:11Z | |
| dc.date.available | 2018-03-29T00:16:11Z | |
| dc.identifier | Journal Of Chromatography B-analytical Technologies In The Biomedical And Life Sciences. Elsevier Science Bv, v. 848, n. 2, n. 311, n. 316, 2007. | |
| dc.identifier | 1570-0232 | |
| dc.identifier | WOS:000245762600019 | |
| dc.identifier | 10.1016/j.jchromb.2006.10.045 | |
| dc.identifier | http://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79551 | |
| dc.identifier | http://www.repositorio.unicamp.br/handle/REPOSIP/79551 | |
| dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/79551 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1285216 | |
| dc.description | A simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of memantine (1) in human plasma is presented. Sample preparation consisted of the addition of amantadine (11) as internal standard (IS), liquid-liquid extraction in basic conditions using a mixture of diethyl ether-chloroform (7:3, v/v) as extracting solvent, followed by centrifugation, solvent evaporation and sample reconstitution in methanol. Both I and 11 (internal standard) were analyzed using a C18 column and a mobile phase composed of methanol-water-formic acid (80:20:0.1, v/v/v). Eluted compounds were monitored using positive mode electrospray (ES) tandem mass spectrometry. The analyses were carried out by selected reaction monitoring (SRM) using the parent to daughter combinations of m/z 180 > 163 (memantine) and m/z 152 > 135 (amantadine). The peak areas from the analyte and IS were used for quantification of I. The achieved limit of quantification (LOQ) was 0.1 ng/mL; the assay exhibited a linear dynamic range of 0.1-50.0 ng/mL with a determination coefficient (r(2)) of at least 0.98. Validation results on linearity, specificity, accuracy, precision and stability, as well as on application to the analysis of samples taken up to 320 h after oral administration of 20 mg (two 10 mg capsules) of I in healthy volunteers demonstrated the applicability to bioequivalence studies. (c) 2006 Elsevier B.V. All rights reserved. | |
| dc.description | 848 | |
| dc.description | 2 | |
| dc.description | 311 | |
| dc.description | 316 | |
| dc.language | en | |
| dc.publisher | Elsevier Science Bv | |
| dc.publisher | Amsterdam | |
| dc.publisher | Holanda | |
| dc.relation | Journal Of Chromatography B-analytical Technologies In The Biomedical And Life Sciences | |
| dc.relation | J. Chromatogr. B | |
| dc.rights | fechado | |
| dc.rights | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
| dc.source | Web of Science | |
| dc.subject | mass spectrometry | |
| dc.subject | bioequivalence | |
| dc.subject | memantine | |
| dc.subject | Validation | |
| dc.subject | Urine | |
| dc.title | Determination of memantine in human plasma by liquid chromatography-electrospray tandem mass spectrometry: Application to a bioequivalence study | |
| dc.type | Artículos de revistas | |
