Effects of pH and temperature on the chromatographic performance and stability of immobilized poly(methyloctylsiloxane) stationary phases

dc.creatorBorges, EM
dc.creatorCollins, CH
dc.date2012
dc.date37316
dc.date2014-07-30T17:21:41Z
dc.date2015-11-26T17:28:49Z
dc.date2014-07-30T17:21:41Z
dc.date2015-11-26T17:28:49Z
dc.date.accessioned2018-03-29T00:15:54Z
dc.date.available2018-03-29T00:15:54Z
dc.identifierJournal Of Chromatography A. Elsevier Science Bv, v. 1227, n. 174, n. 180, 2012.
dc.identifier0021-9673
dc.identifierWOS:000301563700020
dc.identifier10.1016/j.chroma.2012.01.001
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/65047
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/65047
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1285142
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionThe effects of mobile phase pH, temperature, buffer type and buffer concentration on the selectivity and stability of four stationary phases, with different PMOS loadings, prepared by the thermal immobilization of poly(methyloctylsiloxane) on to silica (PMOS-SiO2), were evaluated with both hydrophobic and hydrophilic basic solutes. These solutes show longer retention times at near neutral pH, where both the silanols and the basic solutes are partially ionized, and shorter retention times in more alkaline pH, where the silanols are mostly ionized and the basic solutes are not ionized. Increases in temperature and buffer concentration also result in shorter retention times. These PMOS-SiO2 stationary phases are quite stable at low pH and are also stable at ambient temperature (23 degrees C) using pH 7 phosphate. The PMOS-SiO2 stationary phases are more stable at higher pH using triethylamine (pH 11) and borate (pH 10) buffers than with phosphate and carbonate buffers. Temperature increases stationary phase degradation, while buffer concentration has a minimal effect on stationary phase degradation, indicating that these PMOS-SiO2 stationary phases have stabilities similar to the equivalent chemically bonded phases. (C) 2012 Elsevier B.V. All rights reserved.
dc.description1227
dc.description174
dc.description180
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationJournal Of Chromatography A
dc.relationJ. Chromatogr. A
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectBasic solutes
dc.subjectChemical and thermal stability
dc.subjectIon-exchange
dc.subjectRP-HPLC
dc.subjectSilanol activity
dc.subjectPolybutadiene-coated Zirconia
dc.subjectReversed-phase
dc.subjectLiquid-chromatography
dc.subjectIonizable Compounds
dc.subjectMobile-phase
dc.subjectRetention
dc.subjectExchange
dc.subjectMethanol
dc.subjectSilica
dc.subjectSeparations
dc.titleEffects of pH and temperature on the chromatographic performance and stability of immobilized poly(methyloctylsiloxane) stationary phases
dc.typeArtículos de revistas


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