Opening of the mitochondrial permeability transition pore by uncoupling or inorganic phosphate in the presence of Ca2+ is dependent on mitochondrial-generated reactive oxygen species

dc.creatorKowaltowski, AJ
dc.creatorCastilho, RF
dc.creatorVercesi, AE
dc.date1996
dc.date39448
dc.date2014-12-02T16:27:06Z
dc.date2015-11-26T17:28:47Z
dc.date2014-12-02T16:27:06Z
dc.date2015-11-26T17:28:47Z
dc.date.accessioned2018-03-29T00:15:52Z
dc.date.available2018-03-29T00:15:52Z
dc.identifierFebs Letters. Elsevier Science Bv, v. 378, n. 2, n. 150, n. 152, 1996.
dc.identifier0014-5793
dc.identifierWOS:A1996TP29900010
dc.identifier10.1016/0014-5793(95)01449-7
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69479
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/69479
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69479
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1285133
dc.descriptionIn this study, we show that mitochondrial membrane permeability transition in Ca2+-loaded mitochondria treated with carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone (FCCP) or inorganic phosphate (P-i) is preceded by enhanced production of H2O2. This production is inhibited either by ethylene glycol-bis(b-aminoethyl ether)N,N,N',N'-tetraacetic acid (EGTA) or Mg2+, but not by cyclosporin A, Permeability transition is prevented either by EGTA, catalase or dithiothreitol, suggesting the involvement of Ca2+, H2O2 and oxidation of membrane protein thiols in this mechanism, When mitochondria are incubated under anaerobiosis, no permeabilization or H2O2 production occurs, Based on these results we conclude that mitochondrial permeability transition induced by P-i or FCCP-uncoupling is dependent on mitochondrial-generated reactive oxygen species.
dc.description378
dc.description2
dc.description150
dc.description152
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationFebs Letters
dc.relationFEBS Lett.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectmitochondrial permeability transition pore
dc.subjectcalcium
dc.subjectreactive oxygen species
dc.subjectPlus Prooxidants
dc.subjectOxidative Stress
dc.subjectMembrane
dc.subjectParticipation
dc.subjectModulation
dc.titleOpening of the mitochondrial permeability transition pore by uncoupling or inorganic phosphate in the presence of Ca2+ is dependent on mitochondrial-generated reactive oxygen species
dc.typeArtículos de revistas


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