Bacterial examination of endodontic infections by clonal analysis in concert with denaturing high-performance liquid chromatography

dc.creatorJacinto, RC
dc.creatorGomes, BPFA
dc.creatorDesai, M
dc.creatorRajendram, D
dc.creatorShah, HN
dc.date2007
dc.dateDEC
dc.date2014-11-17T10:25:59Z
dc.date2015-11-26T17:26:18Z
dc.date2014-11-17T10:25:59Z
dc.date2015-11-26T17:26:18Z
dc.date.accessioned2018-03-29T00:13:30Z
dc.date.available2018-03-29T00:13:30Z
dc.identifierOral Microbiology And Immunology. Blackwell Publishing, v. 22, n. 6, n. 403, n. 410, 2007.
dc.identifier0902-0055
dc.identifierWOS:000250806700008
dc.identifier10.1111/j.1399-302X.2007.00378.x
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55277
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/55277
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55277
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1284528
dc.descriptionBackground/aims: The aim of this study was to examine the diversity of bacterial species in the infected root canals of teeth associated with endodontic abscesses by cloning and sequencing techniques in concert with denaturing high-performance liquid chromatography. Methods: Samples collected from five infected root canals were subjected to polymerase chain reaction (PCR) with universal 16S ribosomal DNA primers. Products of these PCRs were cloned and sequenced. Denaturing high-performance liquid chromatography (DHPLC) was used as a screening method to reduce the number of clones necessary for DNA sequencing. Results: All samples were positive for the presence of bacteria and a range of 7-13 different bacteria were found per root canal sample. In total, 48 different oral clones were detected among the five root canal samples. Olsenella profusa was the only species present in all samples. Porphyromonas gingivalis, Dialister pneumosintes, Dialister invisus, Lachnospiraceae oral clone, Staphylococcus aureus, Pseudoramibacter alactolyticus, Peptostreptococcus micros and Enterococcus faecalis were found in two of the five samples. The majority of the taxa were present in only one sample, for example Tannerella forsythia, Shuttleworthia satelles and Filifactor alocis. Some facultative anaerobes that are frequently isolated from endodontic infections such as E. faecalis, Streptococcus anginosus and Lactobacillus spp. were also found in this study. Conclusion: Clonal analysis of the microflora associated with endodontic infections revealed a wide diversity of oral species.
dc.description22
dc.description6
dc.description403
dc.description410
dc.languageen
dc.publisherBlackwell Publishing
dc.publisherOxford
dc.publisherInglaterra
dc.relationOral Microbiology And Immunology
dc.relationOral Microbiol. Immunol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectanaerobic bacteria
dc.subjectclonal analysis
dc.subjectDHPLC
dc.subjectendodontic infections
dc.subjectDental Root Canals
dc.subjectHuman Oral-cavity
dc.subjectMolecular Analysis
dc.subjectAntimicrobial Susceptibility
dc.subjectDialister-pneumosintes
dc.subjectEnterococcus-faecalis
dc.subjectNested Pcr
dc.subjectGen. Nov.
dc.subjectIdentification
dc.subjectMicroorganisms
dc.titleBacterial examination of endodontic infections by clonal analysis in concert with denaturing high-performance liquid chromatography
dc.typeArtículos de revistas


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