The phosphatidylinositol/AKT/atypical PKC pathway is involved in the improved insulin sensitivity by DHEA in muscle and liver of rats in vivo

dc.creatorCampbell, CSG
dc.creatorCaperuto, LC
dc.creatorHirata, AE
dc.creatorAraujo, EP
dc.creatorVelloso, LA
dc.creatorSaad, MJ
dc.creatorCarvalho, CRO
dc.date2004
dc.date43770
dc.date2014-11-16T18:14:04Z
dc.date2015-11-26T17:26:01Z
dc.date2014-11-16T18:14:04Z
dc.date2015-11-26T17:26:01Z
dc.date.accessioned2018-03-29T00:13:12Z
dc.date.available2018-03-29T00:13:12Z
dc.identifierLife Sciences. Pergamon-elsevier Science Ltd, v. 76, n. 1, n. 57, n. 70, 2004.
dc.identifier0024-3205
dc.identifierWOS:000224825700007
dc.identifier10.1016/j.lfs.2004.06.017
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/72233
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/72233
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/72233
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1284454
dc.descriptionDHEA improves insulin sensitivity and has anti-obesity effect in animal models and men. However, the molecular mechanisms by which DHEA improves insulin action have not been clearly understood. In the present study, we examined the protein levels and phosphorylation state of insulin receptor (IR), IRS-1 and IRS-2, the association between IRSs and PI3K and SHP2, the insulin-induced IRSs associated PI 3-kinase activities, and the phosphorylation status of AKT and atypical PKCzeta/lambda in the liver and the muscle of 6 month-old Wistar rats treated with DHEA. There was no change in IR, IRS-1 and IRS-2 protein levels in both tissues of treated rats analysed by immunoblotting. On the other hand, insulin-induced, IRS-1 tyrosine phosphorylation was increased in both tissues While IRS-2 tyrosyl phosphorylation was increased in liver of DHEA treated group. The PI3-kinase/AKT pathway was increased in the liver and the PI3K/atypical PKCzeta/lambda pathway was increased in the muscle of DHEA treated rats. These data indicate that these regulations of early steps of insulin action may play a role in the intracellular mechanism for the improved insulin sensitivity observed in this animal model. (C) 2004 Elsevier Inc. All rights reserved.
dc.description76
dc.description1
dc.description57
dc.description70
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationLife Sciences
dc.relationLife Sci.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectinsulin action
dc.subjectDHEA
dc.subjectIRS-1
dc.subjectIRS-2
dc.subjectDehydroepiandrosterone-sulfate Levels
dc.subjectReceptor Substrate-1
dc.subjectSignal-transduction
dc.subjectGlucose-transport
dc.subjectHypertensive Men
dc.subjectResistant Obese
dc.subjectIntact Rat
dc.subjectMice
dc.subjectIrs-1
dc.subjectDisruption
dc.titleThe phosphatidylinositol/AKT/atypical PKC pathway is involved in the improved insulin sensitivity by DHEA in muscle and liver of rats in vivo
dc.typeArtículos de revistas


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