Detection of herpesvirus type 8 (HHV8) in children's tonsils and adenoids by immunohistochemistry and in situ hybridization

dc.creatorChagas, CA
dc.creatorEndo, LH
dc.creatorSakano, E
dc.creatorPinto, GA
dc.creatorBrousset, P
dc.creatorVassallo, J
dc.date2006
dc.dateJAN
dc.date2014-11-16T16:47:02Z
dc.date2015-11-26T17:25:55Z
dc.date2014-11-16T16:47:02Z
dc.date2015-11-26T17:25:55Z
dc.date.accessioned2018-03-29T00:13:07Z
dc.date.available2018-03-29T00:13:07Z
dc.identifierInternational Journal Of Pediatric Otorhinolaryngology. Elsevier Ireland Ltd, v. 70, n. 1, n. 65, n. 72, 2006.
dc.identifier0165-5876
dc.identifierWOS:000234767800010
dc.identifier10.1016/j.ijporl.2005.04.030
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/61309
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/61309
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/61309
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1284433
dc.descriptionObjective: Human herpesvirus 8 (HHV8) has been associated with multicentric Castleman's disease, Kaposi's sarcoma and effusion non-Hodgkin's lymphoma. Epidemiotogical studies have shown seropositivity in variable proportions of populations. It seems to be sexually transmitted among adults and through oral contact among children. The virus has been demonstrated in desquamating oral epithelial cells, but there is no report on its presence in the Waldeyer's ring. The purpose of the present study is to detect HHV8 in tonsils and adenoids from children up to 20 years of age in which these organs had been surgically removed due to hypertrophy, using immunohistochemistry and in situ hybridization. Methods: Paraffin wax-embedded sections consisting of 181 tonsils and 162 adenoids from 293 patients were analyzed. HHV8 was detected by immunohistochemistry (IHC) using the anti-LNA1 antibody (Novocastra) and the LSAB+ detection system (Dako). For the in situ hybridization (ISH), the T1-1 probe for the viral mRNA and the detection system used were provided by Novocastra. Results: In 20 cases (6.83%), HHV8 was detected in cells morphologically characterized as lymphoid. In three of them epithelial cells were also positive. In 19 cases, the virus was detected in tonsils and in just 1 case in an adenoid. In all 20 cases detection was possible by ISH, whereas in only 2 of them there was a concomitant positivity by IHC. Conclusion: Our data support the oral route of contamination by HHV8 in children, in whom tonsils and adenoids may harbor the virus. It is found especially in tonsils and only rarely in adenoids. In these organs, ISH is the method of choice to detect this virus, probably due to the small amount of viral proteins. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
dc.description70
dc.description1
dc.description65
dc.description72
dc.languageen
dc.publisherElsevier Ireland Ltd
dc.publisherClare
dc.publisherIrlanda
dc.relationInternational Journal Of Pediatric Otorhinolaryngology
dc.relationInt. J. Pediatr. Otorhinolaryngol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjecthuman herpesvirus 8
dc.subjecttonsils
dc.subjectadenoids
dc.subjectimmunohistochemistry
dc.subjectin situ hybridization
dc.subjectSarcoma-associated Herpesvirus
dc.subjectEpstein-barr-virus
dc.subjectMulticentric Castlemans-disease
dc.subjectLatent Nuclear Antigen-1
dc.subjectKaposis-sarcoma
dc.subjectLymphoid-cells
dc.subjectSpindle Cells
dc.subjectHuman-herpesvirus-8
dc.subjectTransmission
dc.subjectAntibodies
dc.titleDetection of herpesvirus type 8 (HHV8) in children's tonsils and adenoids by immunohistochemistry and in situ hybridization
dc.typeArtículos de revistas


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