ERIC- and REP-PCR amplify non-repetitive fragments from the genome of Drechslera avenae and Stemphylium solani

dc.creatorMehta, A
dc.creatorMehta, YR
dc.creatorRosato, YB
dc.date2002
dc.dateMAY 21
dc.date2014-11-16T16:33:04Z
dc.date2015-11-26T17:25:51Z
dc.date2014-11-16T16:33:04Z
dc.date2015-11-26T17:25:51Z
dc.date.accessioned2018-03-29T00:13:04Z
dc.date.available2018-03-29T00:13:04Z
dc.identifierFems Microbiology Letters. Elsevier Science Bv, v. 211, n. 1, n. 51, n. 55, 2002.
dc.identifier0378-1097
dc.identifierWOS:000176280000009
dc.identifier10.1111/j.1574-6968.2002.tb11202.x
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/65552
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/65552
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/65552
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1284416
dc.descriptionIn the present study, the homology relationships among fragments amplified by ERIC (Enterobacterial Repetitive Intergenic Consensus) and REP (Repetitive Extragenic Palindromic) primers in Drechslera avenae and Stemphylium solani causing leaf blotch and black spot of oats and leaf blight of cotton, respectively, were investigated by Southern hybridization followed by DNA sequencing. ERIC- and REP-PCR were performed for 24 isolates of D. avenae and 33 isolates of S. solani. The banding patterns obtained were transferred to nylon membranes and a total of 16 fragments, four ERIC and four REP bands from each fungus, used as probes. The hybridization and DNA sequencing revealed a low degree of similarity among the bands indicating that the regions amplified by these primers are not repetitive and that the primers anneal at random regions in the genomes of D. avenae and S. solani. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.
dc.description211
dc.description1
dc.description51
dc.description55
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationFems Microbiology Letters
dc.relationFEMS Microbiol. Lett.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectERIC- and REP-PCR
dc.subjectgenetic diversity
dc.subjectDrechslera avenae
dc.subjectStemphylium solani
dc.subjectSequences
dc.titleERIC- and REP-PCR amplify non-repetitive fragments from the genome of Drechslera avenae and Stemphylium solani
dc.typeArtículos de revistas


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