A survey of mutations in the Cochliomyia hominivorax (Diptera : Calliphoridae) esterase E3 gene associated with organophosphate resistance and the molecular identification of mutant alleles

dc.creatorde Carvalho, RA
dc.creatorTorres, TT
dc.creatorde Azeredo-Espin, AML
dc.date2006
dc.dateSEP 10
dc.date2014-11-16T15:39:05Z
dc.date2015-11-26T17:25:41Z
dc.date2014-11-16T15:39:05Z
dc.date2015-11-26T17:25:41Z
dc.date.accessioned2018-03-29T00:12:54Z
dc.date.available2018-03-29T00:12:54Z
dc.identifierVeterinary Parasitology. Elsevier Science Bv, v. 140, n. 41732, n. 344, n. 351, 2006.
dc.identifier0304-4017
dc.identifierWOS:000240398600020
dc.identifier10.1016/j.vetpar.2006.04.010
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/53871
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/53871
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/53871
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1284374
dc.descriptionCochliomyia hominivorax (Calliphoridae) is one of the most important myiasis-causing flies and is responsible for severe economic losses to the livestock industry throughout the Neotropical region. In Brazil, C. hominivorax has been controlled mainly with organophosphate (OP) insecticides, although the inappropriate use of these chemicals can result in the selection of resistant flies. Changes in carboxylesterase activity have been associated with OP insecticides in some arthopodan species. In this work, we isolated and characterized part of the E3 gene in C. hominivorax (Ch alpha E7), which contained the same substitutions responsible for the acquisition of OP hydrolase activity in Lucilia cuprina (Calliphoridae). Digestion of the polymerase chain reaction products with a restriction enzyme that specifically recognized the mutation site unambiguously differentiated wild and mutated esterase alleles. The PCR-RFLP assay therefore provided a fast, reliable DNA-based method for identifying C. hominivorax individuals with a mutation in the esterase gene. Further bioassays to determine the association of this mutation with OP resistance in C. hominivorax should allow the development of more effective strategies for managing this species. (c) 2006 Elsevier B.V. All rights reserved.
dc.description140
dc.description41732
dc.description344
dc.description351
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationVeterinary Parasitology
dc.relationVet. Parasitol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectcarboxylesterase
dc.subjectinsecticide resistance
dc.subjectmyiasis
dc.subjectNew World screw-worm
dc.subjectpest management
dc.subjectPCR-RFLP
dc.subjectAmino-acid Substitution
dc.subjectLucilia-cuprina
dc.subjectInsecticide Resistance
dc.subjectDrosophila-melanogaster
dc.subjectMalathion
dc.subjectCarboxylesterases
dc.subjectBiochemistry
dc.subjectHydrolase
dc.subjectMechanism
dc.subjectProtein
dc.titleA survey of mutations in the Cochliomyia hominivorax (Diptera : Calliphoridae) esterase E3 gene associated with organophosphate resistance and the molecular identification of mutant alleles
dc.typeArtículos de revistas


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