Propagation of avian metapneumovirus subtypes A and B using chicken embryo related and other cell systems

dc.creatorCoswig, LT
dc.creatordos Santos, MB
dc.creatorHafez, HM
dc.creatorFerreira, HL
dc.creatorArns, CW
dc.date2010
dc.dateJUL
dc.date2014-11-16T14:59:39Z
dc.date2015-11-26T17:25:18Z
dc.date2014-11-16T14:59:39Z
dc.date2015-11-26T17:25:18Z
dc.date.accessioned2018-03-29T00:12:34Z
dc.date.available2018-03-29T00:12:34Z
dc.identifierJournal Of Virological Methods. Elsevier Science Bv, v. 167, n. 1, n. 1, n. 4, 2010.
dc.identifier0166-0934
dc.identifierWOS:000278782700001
dc.identifier10.1016/j.jviromet.2010.02.018
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/70525
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/70525
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/70525
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1284291
dc.descriptionPrimary isolation of avian metapneumovirus (aMPV) is carried out using tracheal organ culture (TOC) or chicken embryonated eggs with subsequent adaptation in chicken embryo fibroblasts (CEF) or Vero cultures. This study was conducted to evaluate six different cell lines and two avian culture systems for the propagation of aMPV subtypes A and B. The chicken embryo related (CER) cells were used successfully for primary isolation. In addition to Vero and baby hamster kidney (BHK-21) cells, CER cells were also shown to be the most appropriate for propagation of aMPV considering high titres. Propagation of A and B subtypes in CEF and TOC remained efficient after the primary isolation and several passages of viruses in the CER cell line. The growth curves were created using CER, Vero and BHK-21 cell lines. Compared with growth, both yielded higher titres in CER cells during the first 30 h after infection, but no significant difference was observed in the results obtained from CER and Vero cells. This data show that CER cells are adequate for aMPV subtypes A and B propagation, giving similar results to Vero cells. (C) 2010 Elsevier B.V. All rights reserved.
dc.description167
dc.description1
dc.description1
dc.description4
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationJournal Of Virological Methods
dc.relationJ. Virol. Methods
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectAvian metapneumovirus
dc.subjectCell propagation
dc.subjectCER
dc.subjectSwollen Head Syndrome
dc.subjectTurkey Rhinotracheitis
dc.subjectPneumovirus
dc.subjectInfection
dc.subjectVirus
dc.subjectSusceptibility
dc.subjectVaccine
dc.subjectLines
dc.subjectAssay
dc.subjectReplication
dc.titlePropagation of avian metapneumovirus subtypes A and B using chicken embryo related and other cell systems
dc.typeArtículos de revistas


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