Protein L-agarose for adsorption of autoantibodies: A potential tool for extracorporeal treatment

dc.creatorDuarte, SS
dc.creatorZollner, RD
dc.creatorBueno, SMA
dc.date2005
dc.dateAPR
dc.date2014-11-16T13:13:11Z
dc.date2015-11-26T17:25:06Z
dc.date2014-11-16T13:13:11Z
dc.date2015-11-26T17:25:06Z
dc.date.accessioned2018-03-29T00:12:23Z
dc.date.available2018-03-29T00:12:23Z
dc.identifierArtificial Organs. Blackwell Publishing Inc, v. 29, n. 4, n. 313, n. 323, 2005.
dc.identifier0160-564X
dc.identifierWOS:000228185200005
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/58328
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/58328
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/58328
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1284246
dc.descriptionThis work investigated the adsorption of autoantibodies such as anti-SS-A/Ro, anti-SS-B/La, anti-Sm, and anti-dsDNA on protein L-agarose gel. In order to determine better conditions for IgG adsorption on this matrix, some buffer systems were tested. Adsorption data were analyzed using the Langmuir and Langmuir-Freundlich isotherm models. The experimental isotherms were best described by the Langmuir-Freundlich model, which indicated negative and positive cooperativities for binding in the presence of PBS and HEPES buffers, respectively. The K-d values for phosphate buffered saline solution (PBS) and hydroxyethylpiperazine ethanesulfonic acid (HEPES) were 2.8 x 10(-7) M and 3.2 x 10(-7) M, respectively, which indicate a high affinity between IgG and the immobilized protein L. The amount of protein adsorbed per amount of protein loaded was high for anti-Sm (44%) and anti-dsDNA (46%), but low for anti-SS-B/La (9%). The amount of albumin adsorbed was lower than 0.06 mg/mL, which may remove the need for a plasma replacement solution in clinical apheresis.
dc.description29
dc.description4
dc.description313
dc.description323
dc.languageen
dc.publisherBlackwell Publishing Inc
dc.publisherMalden
dc.publisherEUA
dc.relationArtificial Organs
dc.relationArtif. Organs
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectimmunoglobulin
dc.subjectapheresis
dc.subjectadsorption
dc.subjectautoantibodies
dc.subjectprotein L
dc.subjectSystemic-lupus-erythematosus
dc.subjectPeptostreptococcus-magnus
dc.subjectA Immunoadsorption
dc.subjectMyasthenia-gravis
dc.subjectSjogrens-syndrome
dc.subjectBinding
dc.subjectPurification
dc.titleProtein L-agarose for adsorption of autoantibodies: A potential tool for extracorporeal treatment
dc.typeArtículos de revistas


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