Acid phosphatase activities during the germination of Glycine max seeds

dc.creatordos Prazeres, JN
dc.creatorFerreira, CV
dc.creatorAoyama, H
dc.date2004
dc.dateJAN
dc.date2014-11-16T11:37:39Z
dc.date2015-11-26T17:24:42Z
dc.date2014-11-16T11:37:39Z
dc.date2015-11-26T17:24:42Z
dc.date.accessioned2018-03-29T00:11:59Z
dc.date.available2018-03-29T00:11:59Z
dc.identifierPlant Physiology And Biochemistry. Editions Scientifiques Medicales Elsevier, v. 42, n. 1, n. 15, n. 20, 2004.
dc.identifier0981-9428
dc.identifierWOS:000189150800003
dc.identifier10.1016/j.plaphy.2003.10.009
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/54109
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/54109
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/54109
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1284144
dc.descriptionIn this paper, we describe a study concerning the determination of some characteristics of soybean seedlings and the detection of acid phosphatase activities towards different substrates during the germination. Enzyme activities with p-nitrophenylphosphate (PNPP) and inorganic pyrophosphate (PPi) as substrates were detected from the 5th and 7th days after germination, respectively. Acid phosphatase activities with tyrosine phosphate (TyrP), glucose-6-phosphate (G6P) and phosphoenol pyruvate (PEP) were also observed but to a lesser extent. Under the same conditions, no enzyme activity was detected with phytic acid (PhyAc) as substrate. The appearance of phosphatase activity was coincident with the decrease of inorganic phosphate content during germination; over the same period, the protein content increased up to the 5th day, decreased until the 8th day, and remained constant after this period. Relative to phosphatase activity in the cotyledons. the activities detected in the hypocotyl and roots were 82% and 38%, respectively. During storage the enzyme maintained about 63% of its activity for 3 months at 5 degreesC. The specificity constant (Vmax/Km) values for pNPP and PPi were 212 and 64 mukat mM(-1) mg(-1), respectively. Amongst the substrates tested, PPi could be a potential physiological substrate for acid phosphatase during the germination of soybean seeds. (C) 2003 Elsevier SAS. All rights reserved.
dc.description42
dc.description1
dc.description15
dc.description20
dc.languageen
dc.publisherEditions Scientifiques Medicales Elsevier
dc.publisherParis
dc.publisherFrança
dc.relationPlant Physiology And Biochemistry
dc.relationPlant Physiol. Biochem.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectacid phosphatase
dc.subjectgermination
dc.subjectGlycine max
dc.subjectphysiological substrate
dc.subjectsoybean seeds
dc.subjectMultiple Forms
dc.subjectSoybean Seeds
dc.subjectPhytic Acid
dc.subjectPurification
dc.subjectPhytase
dc.subjectCotyledons
dc.subjectPhosphorus
dc.subjectSeedlings
dc.subjectLocalization
dc.subjectFeedstuffs
dc.titleAcid phosphatase activities during the germination of Glycine max seeds
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución