Inhibition of mitochondrial permeability transition by low pH is associated with less extensive membrane protein thiol oxidation

dc.creatorTeixeira, BMAR
dc.creatorKowaltowski, AJ
dc.creatorCastilho, RF
dc.creatorVercesi, AE
dc.date1999
dc.dateDEC
dc.date2014-12-02T16:29:48Z
dc.date2015-11-26T17:24:37Z
dc.date2014-12-02T16:29:48Z
dc.date2015-11-26T17:24:37Z
dc.date.accessioned2018-03-29T00:11:55Z
dc.date.available2018-03-29T00:11:55Z
dc.identifierBioscience Reports. Kluwer Academic/plenum Publ, v. 19, n. 6, n. 525, n. 533, 1999.
dc.identifier0144-8463
dc.identifierWOS:000087277700002
dc.identifier10.1023/A:1020210827389
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/80591
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/80591
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/80591
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1284128
dc.descriptionCa2+ and inorganic phosphate-induced mitochondrial swelling and membrane protein thiol oxidation, which are associated with mitochondrial permeability transition, are inhibited by progressively decreasing the incubation medium pH between 7.2 and 6.0. Nevertheless, the detection of mitochondrial H2O2 production under these conditions is increased. Permeability transition induced by phenylarsine oxide, which promotes membrane protein thiol cross-linkage in a process independent of Ca2+ or reactive oxygen species, is also strongly inhibited in acidic incubation media. In addition, we observed that the decreased protein thiol reactivity with phenylarsine oxide or phenylarsine oxide-induced swelling at pH 6.0 is reversed by diethyl pyrocarbonate, in a hydroxylamine-sensitive manner. These results provide evidence that the inhibition of mitrochondrial permeability transition observed at lower incubation medium pH is mediated by a decrease in membrane protein thiol reactivity, related to the protonation of protein histidyl residues.
dc.description19
dc.description6
dc.description525
dc.description533
dc.languageen
dc.publisherKluwer Academic/plenum Publ
dc.publisherNew York
dc.publisherEUA
dc.relationBioscience Reports
dc.relationBiosci. Rep.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectmitochondrial permeability transition
dc.subjectacid pH
dc.subjectprotein sulfhydryl oxidation
dc.subjectcalcium
dc.subjectreactive oxygen species
dc.subjectCell-death
dc.subjectRat-liver
dc.subjectSuperoxide
dc.subjectMechanism
dc.subjectPore
dc.subjectReperfusion
dc.subjectApoptosis
dc.subjectCalcium
dc.subjectStress
dc.subjectInjury
dc.titleInhibition of mitochondrial permeability transition by low pH is associated with less extensive membrane protein thiol oxidation
dc.typeArtículos de revistas


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