Estimation of the fractional sarcoplasmic reticulum Ca2+ release in intact cardiomyocytes using integrated Ca2+ fluxes

dc.creatorBassani, RA
dc.creatorRicardo, RA
dc.creatorBassani, JWM
dc.date2012
dc.dateDEC
dc.date2014-11-16T10:48:49Z
dc.date2015-11-26T17:24:29Z
dc.date2014-11-16T10:48:49Z
dc.date2015-11-26T17:24:29Z
dc.date.accessioned2018-03-29T00:11:47Z
dc.date.available2018-03-29T00:11:47Z
dc.identifierGeneral Physiology And Biophysics. General Physiol And Biophysics, v. 31, n. 4, n. 401, n. 408, 2012.
dc.identifier0231-5882
dc.identifierWOS:000311981300006
dc.identifier10.4149/gpb_2012_042
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/65656
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/65656
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/65656
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1284093
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionThe sarcoplasmic reticulum (SR) is the main source of contraction-activating Ca2+ in the adult mammalian myocardium. The fraction of the SR Ca2+ content released at a twitch (fractional SR Ca2+ release, FR) is an important parameter for assessing the efficiency of excitation-contraction coupling under physiological and pathophysiological conditions, as well as for identification of modulators of this process. We here describe and propose an approach for FR quantitation based on the estimation of integrated Ca2+ fluxes mediated by different transporters that remove the ion from the cytosol. These fluxes may be calculated solely from the measurement of cytosolic free Ca2+ concentration ([Ca2+](i)) during Ca2+ transients evoked under selective inhibition of the transporters, and from the cell Ca2+ buffering parameters available in the literature. The FR values obtained with this approach in intact rat ventricular myocytes (0.63 +/- 0.04; n = 12) were comparable to those estimated in the same cell type with an already established method, based on electrophysiological measurements with the patch-clamp technique, in addition to [Ca2+](i); measurement (0.69 +/- 0.05; n = 6; p > 0.40). We conclude that the proposed method might be a suitable and a technically simpler alternative to the electrophysiological method for FR estimation.
dc.description31
dc.description4
dc.description401
dc.description408
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCNPq [Proc N. 300632/2005-3, 141175/2002-8]
dc.descriptionFAPESP [Proc. N 2006/05717-7, 2008/54795-6]
dc.languageen
dc.publisherGeneral Physiol And Biophysics
dc.publisherBratislava
dc.publisherEslováquia
dc.relationGeneral Physiology And Biophysics
dc.relationGen. Physiol. Biophys.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectSarcoplasmic reticulum
dc.subjectFractional calcium release
dc.subjectExcitation-contraction coupling
dc.subjectMyocardium
dc.subjectRabbit Ventricular Myocytes
dc.subjectRat Cardiac Myocytes
dc.subjectCalcium-concentration
dc.subjectMouse Cardiomyocytes
dc.subjectCellular Mechanisms
dc.subjectProtein-kinase
dc.subjectRelaxation
dc.subjectMyocardium
dc.subjectHeart
dc.subjectOverexpression
dc.titleEstimation of the fractional sarcoplasmic reticulum Ca2+ release in intact cardiomyocytes using integrated Ca2+ fluxes
dc.typeArtículos de revistas


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