The rus operon genes are differentially regulated when Acidithiobacillus ferrooxidans LR is kept in contact with metal sulfides

dc.creatorCarlos, C
dc.creatorReis, FC
dc.creatorVicentini, R
dc.creatorMadureira, DJ
dc.creatorOttoboni, LMM
dc.date2008
dc.dateOCT
dc.date2014-11-16T06:57:35Z
dc.date2015-11-26T17:23:41Z
dc.date2014-11-16T06:57:35Z
dc.date2015-11-26T17:23:41Z
dc.date.accessioned2018-03-29T00:11:00Z
dc.date.available2018-03-29T00:11:00Z
dc.identifierCurrent Microbiology. Springer, v. 57, n. 4, n. 375, n. 380, 2008.
dc.identifier0343-8651
dc.identifierWOS:000258654500018
dc.identifier10.1007/s00284-008-9208-7
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/72422
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/72422
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/72422
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1283890
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionAcidithiobacillus ferrooxidans is a Gram-negative bacterium that obtains energy from the oxidation of ferrous iron or reduced sulfur compounds. In this bacterium, the proteins encoded by the rus operon are involved in electron transfer from Fe(II) to O(2), and the first two proteins in this pathway also participate in the electron transfer pathway from Fe(II) to NAD(P). In this work we analyzed the expression, by real-time PCR, of the eight genes from the rus operon when A. ferrooxidans LR was grown in the presence of iron (control) and then kept in contact with chalcopyrite (CuFeS(2)) and covellite (CuS). A small decrease in rus operon gene expression was observed in the presence of chalcopyrite, while in the presence of covellite the expression of these genes showed a remarkable decrease. These results can be explained by the absence of ferrous iron in covellite. To explain the expression difference observed between the gene cyc1 and the gene rus, we investigated the information content presented at the Translation Initiation Site (TIS) of both genes. cyc1 showed a highly information content (8.4 bits) that can maximize translation, and rus showed a less favorable context (5.5 bits). Our hypothesis is that the energetic metabolism in A. ferrooxidans may be controlled at the transcriptional and posttranscriptional level by different mechanisms.
dc.description57
dc.description4
dc.description375
dc.description380
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [2005/51332-7, 06/54433-1, 05/00139-2]
dc.descriptionCNPq [134062/2006-0]
dc.languageen
dc.publisherSpringer
dc.publisherNew York
dc.publisherEUA
dc.relationCurrent Microbiology
dc.relationCurr. Microbiol.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectThiobacillus-ferrooxidans
dc.subjectCytochrome-oxidase
dc.subjectElectron-transfer
dc.subjectProtein-synthesis
dc.subjectBc(1) Complexes
dc.subjectExpression
dc.subjectRusticyanin
dc.subjectInitiation
dc.subjectIron
dc.titleThe rus operon genes are differentially regulated when Acidithiobacillus ferrooxidans LR is kept in contact with metal sulfides
dc.typeArtículos de revistas


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