Evaluation of culture media for counts of Bifidobacterium animalis subsp lactis BB 12 in yoghurt after refrigerated storage

dc.creatorFachin, L
dc.creatorMoryia, J
dc.creatorGandara, AL
dc.creatorViotto, WH
dc.date2008
dc.dateAPR-JUN
dc.date2014-11-16T05:58:16Z
dc.date2015-11-26T17:23:38Z
dc.date2014-11-16T05:58:16Z
dc.date2015-11-26T17:23:38Z
dc.date.accessioned2018-03-29T00:10:57Z
dc.date.available2018-03-29T00:10:57Z
dc.identifierBrazilian Journal Of Microbiology. Soc Brasileira Microbiologia, v. 39, n. 2, n. 357, n. 361, 2008.
dc.identifier1517-8382
dc.identifierWOS:000258097100029
dc.identifier10.1590/S1517-83822008000200029
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/65791
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/65791
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/65791
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1283876
dc.descriptionThe agar RCPB pH5 has been considered a good alternative for counts of Bifidobacterium in yoghurt. However, during the refrigerated storage of yoghurt it is extremely difficult to count this microorganism due to the size of the colonies, which are so small they require the aid of a stereoscope to count them. Another agar, MRS-LP, has been also recommended for counts of Bifidobacterium in the presence of yoghurt bacteria. This study evaluated the supplementation of RCPB pH5 agar with dehydrated liver extract and the salts KH(2)PO(4), K(2)HPO(4), FeSO(4)7H(2)O, MnSO(4)H(2)O and MgSO(4)7H(2)O, aiming at improving the differentiation of Bifidobacterium in yoghurt after refrigerated storage, and also evaluated the selective count of Bifidobacterium in yoghurt using the agar MRS-LP. The agar MRS-LP presented the same cell recovery as non-fortified RCPB pH5 agar, used as a standard medium, thus being considered a good option for counts of Bifidobacterium in yoghurt. The fortified RCPB pH5 also presented the same recovery as the standard RCPB pH5 medium, however, the addition of dehydrated liver extract to the RCPB pH5 agar considerably increased the size of the Bifidobacterium colonies after refrigerated storage, making differentiation of the colonies much easier and reliable when compared to the standard non-fortified RPCP pH5. The addition of the salts (KH(2)PO(4), K(2)HPO(4), FeSO(4)7H(2)O, MnSO4H2O and MgSO(4)7H(2)O) had no influence on the performance of the RCPB pH5 agar.
dc.description39
dc.description2
dc.description357
dc.description361
dc.languageen
dc.publisherSoc Brasileira Microbiologia
dc.publisherSao Paulo
dc.publisherBrasil
dc.relationBrazilian Journal Of Microbiology
dc.relationBraz. J. Microbiol.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectcount
dc.subjectBifidobacterium
dc.subjectyoghurt
dc.subjectrefrigerated storage
dc.subjectEnumeration
dc.subjectBacteria
dc.titleEvaluation of culture media for counts of Bifidobacterium animalis subsp lactis BB 12 in yoghurt after refrigerated storage
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución