Application of preparative high-speed counter-current chromatography for the separation of flavonoids from the leaves of Byrsonima crassa Niedenzu (IK)

dc.creatorSannomiya, M
dc.creatorRodrigues, CM
dc.creatorCoelho, RG
dc.creatordos Santos, LC
dc.creatorHiruma-Lima, CA
dc.creatorBrito, ARS
dc.creatorVilegas, W
dc.date2004
dc.dateAPR 30
dc.date2014-11-16T06:04:50Z
dc.date2015-11-26T17:23:36Z
dc.date2014-11-16T06:04:50Z
dc.date2015-11-26T17:23:36Z
dc.date.accessioned2018-03-29T00:10:54Z
dc.date.available2018-03-29T00:10:54Z
dc.identifierJournal Of Chromatography A. Elsevier Science Bv, v. 1035, n. 1, n. 47, n. 51, 2004.
dc.identifier0021-9673
dc.identifierWOS:000220790800007
dc.identifier10.1016/j.chroma.2004.02.050
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/54767
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/54767
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/54767
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1283865
dc.descriptionThe methanolic extract of the leaves of the medicinal plant Byrsonima crassa (Malpighiaceae) contain flavonoids with antioxidant activity. They were separated in a preparative scale using high-speed counter-current chromatography. The optimum solvent system used was composed of a mixture of ethyl acetate-n-propanol-water (140:8:80 (v/v/v)) and led to a successful separation between monoglucosilated flavonoids (quercetin-3-O-alpha-L-arabinoside, quercetin-3-O-beta-D-galactoside) and the biflavonoid amentoflavone in only 3.5 h. The purities of quercetin-3-O-alpha-L-arabinoside (95 mg), quercetin-3-O-beta-D-galactoside (16 mg) and the biflavonoid amentoflavone (114 mg) were all isolated at purity over 95%. Identification was performed by H-1 NMR, C-13 NMR and UV analyses. (C) 2004 Published by Elsevier B.V.
dc.description1035
dc.description1
dc.description47
dc.description51
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationJournal Of Chromatography A
dc.relationJ. Chromatogr. A
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectcounter-current chromatography
dc.subjectByrsonima crassa
dc.subjectplant materials
dc.subjectflavonoids
dc.subjectamentoflavone
dc.subjectCrassifolia
dc.subjectAntioxidant
dc.titleApplication of preparative high-speed counter-current chromatography for the separation of flavonoids from the leaves of Byrsonima crassa Niedenzu (IK)
dc.typeArtículos de revistas


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